Ochieng James Robert, Bachs Marta Planellas, Nsubuga Anthony, Rwego Innocent B, Kisakye John Joseph M, Riba Laura Muro, Figueres Jesus Muro
Department of Zoology, Entomology and Fisheries Sciences, College of Natural Sciences, Makerere University, Kampala, Uganda.
Departament de Medicina I Cirurgia Animals. Universitat Autònoma de Barcelona, Bellaterra, Spain.
J Parasitol Res. 2022 Sep 10;2022:2606871. doi: 10.1155/2022/2606871. eCollection 2022.
Nowadays, despite the instauration of several control strategies, animal trypanosomiasis continues to be reported all over Uganda. Few canine African trypanosomiasis (CAT) studies have been carried out, yet dogs are known reservoirs that share identical home ranges with livestock and serve as parasite link between livestock and humans. This study evaluates the prevalence of CAT in dogs in the Bwindi-Mgahinga and Queen Elizabeth conservation areas. This information will be useful to evaluate the possible role of dogs in the transmission cycle of species in livestock and wild animals. Trypanosome tests using microhematocrit centrifugation/dark ground microscopy technique (MHCT) followed by conventional polymerase chain reaction (cPCR) were performed in blood samples collected from identified indigenous dogs ( = 124). Four (3.23%) out of 124 dogs were positive for CAT. One dog was positive with and three with . . There was no significant statistical difference in CAT prevalence rate in relation to dog's age, sex, and site ( > 0.05). This study reports what we believe is the first time detection of . and . in the indigenous dogs found in the Bwindi-Mgahinga and Queen Elizabeth conservation areas in western Uganda. The noticed . and . could be responsible for both canine and animal trypanosomiasis and represent a serious threat to the livestock industry. Therefore, there is a need for continuous trypanosomiasis surveillance and integrated management in contiguity to wildlife reserves.
如今，尽管已实施了多项防控策略，但乌干达各地仍不断有动物锥虫病的报告。针对犬类非洲锥虫病（CAT）的研究较少，然而犬类是已知的宿主，与牲畜共享相同的栖息地范围，并在牲畜和人类之间充当寄生虫传播的纽带。本研究评估了布温迪-姆加辛加和伊丽莎白女王保护区内犬类感染CAT的流行情况。这些信息将有助于评估犬类在牲畜和野生动物物种传播周期中可能发挥的作用。对从124只已识别的本地犬采集的血液样本，采用微量血细胞比容离心/暗视野显微镜技术（MHCT）进行锥虫检测，随后进行常规聚合酶链反应（cPCR）。124只犬中有4只（3.23%）CAT检测呈阳性。1只犬感染了[具体某种锥虫，原文未完整给出名称]，3只感染了[另一种具体锥虫，原文未完整给出名称]。CAT流行率在犬的年龄、性别和地点方面无显著统计学差异（P>0.05）。本研究报告了我们认为首次在乌干达西部布温迪-姆加辛加和伊丽莎白女王保护区发现的本地犬中检测到[具体锥虫名称，原文未完整给出]。检测到的[具体锥虫名称，原文未完整给出]可能是犬类和动物锥虫病的病因，对畜牧业构成严重威胁。因此，有必要在野生动物保护区附近持续开展锥虫病监测和综合管理。
