Kori Shivpoojan, Sharma Shweta
Toxicology Division, State Forensic Science Laboratory, Gorakhpur, Uttar Pradesh 273001, India.
Institute of Forensic Science & Criminology, Panjab University, Chandigarh 160 014, India.
J Chromatogr Sci. 2022 Dec 29;61(1):96-101. doi: 10.1093/chromsci/bmac074.
A green, simple and cost-effective, validated high-performance liquid chromatographic (HPLC) with UV detector has been developed an analytical method for the simultaneous determination of the most significant phenolic compounds viz. Gallic acid (GA), Quercetin (QC) and Ferulic acid (FA). The detection limit of GA, QC, FA was 0.303, 0.168, 0.0976 μg/mL and quantitation limit was 0.918, 0.051, 0.295 μg/mL, respectively. All parameters of planned validated method were carried out as per the ICH guidelines. This analytical method was applied to determine the phenolic compounds in fruits cultivated in India. Phenolic compounds in fruits extract were quantified using regression equations. This method can be scaled up to determine the alkaloids from several medicinal and poisonous plants samples in pharmaceutical, forensics and industrial areas.
已开发出一种绿色、简单且经济高效的、经验证的配备紫外检测器的高效液相色谱（HPLC）分析方法，用于同时测定最重要的酚类化合物，即没食子酸（GA）、槲皮素（QC）和阿魏酸（FA）。GA、QC、FA的检测限分别为0.303、0.168、0.0976μg/mL，定量限分别为0.918、0.051、0.295μg/mL。计划验证方法的所有参数均按照国际人用药品注册技术协调会（ICH）指南进行。该分析方法用于测定印度种植水果中的酚类化合物。水果提取物中的酚类化合物使用回归方程进行定量。该方法可扩大规模，用于测定制药、法医和工业领域中多种药用和有毒植物样品中的生物碱。
