Department of Pathology and Laboratory Medicine, Children's Mercy Hospital, Kansas City, MO, USA.
Methods Mol Biol. 2022;2546:493-500. doi: 10.1007/978-1-0716-2565-1_44.
Having a diverse gut microbiota has been correlated with the short- and long-term success of allogeneic stem cell transplantation. Intestinal bacteria metabolize the amino acid tryptophan to indole. Indole is further oxidized and sulfonated in the liver to 3-indoxyl sulfate (3-IS), which is then excreted in urine. Urinary 3-IS is a potential biomarker for intestinal health and an early predictor of successful stem cell transplantation. We describe a rapid method for quantifying tryptophan, indole, and 3-indoxyl sulfate in urine specimens, in which urine samples are diluted with a formic acid solution and deuterated internal standards, and then injected on LC-MS/MS for analysis.
肠道微生物多样性与异基因干细胞移植的短期和长期成功相关。肠道细菌将氨基酸色氨酸代谢为吲哚。吲哚在肝脏中进一步氧化和磺化生成 3-吲哚硫酸酯（3-IS），然后从尿液中排出。尿液中的 3-IS 是肠道健康的潜在生物标志物，也是干细胞移植成功的早期预测指标。我们描述了一种快速定量尿液中色氨酸、吲哚和 3-吲哚硫酸酯的方法，该方法将尿液样品用甲酸溶液稀释，并加入氘代内标，然后注入 LC-MS/MS 进行分析。
