Department of Chemistry, Federal University of Amazonas, 69080-900, Manaus, AM, Brazil.
Department of Chemistry, Federal University of São Carlos, 13560-970, São Carlos, SP, Brazil.
Anal Methods. 2022 Oct 13;14(39):3859-3866. doi: 10.1039/d2ay01177h.
A new electrode was prepared based on functionalized graphene and gold nanoparticles dispersed in a chitosan film. Such an electrochemical sensor determines ofloxacin in the presence of dopamine, paracetamol, and caffeine. Characterization (morphological and electrochemical) was done using scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. The sensor design improved the analytical signal, the electrochemical activity, and the electron transfer rate. Ofloxacin was determined by square-wave voltammetry, with a linear concentration range of 0.10-4.9 μmol L ( = 0.999, LOD = 12 nmol L). The proposed sensor showed good repeatability and selectivity and was applied successfully to the determination of ofloxacin in pharmaceutical formulations, synthetic urine, and water river samples. The proposed method proved to be excellent; therefore, it is an alternative method for the determination of ofloxacin.
一种基于功能化石墨烯和金纳米粒子分散在壳聚糖膜中的新型电极被制备出来。这种电化学传感器可在多巴胺、对乙酰氨基酚和咖啡因存在的情况下测定氧氟沙星。采用扫描电子显微镜、电化学阻抗谱和循环伏安法对其进行了形貌和电化学表征。传感器的设计提高了分析信号、电化学活性和电子转移速率。通过方波伏安法测定氧氟沙星，线性浓度范围为 0.10-4.9 μmol L（=0.999，LOD=12 nmol L）。该传感器表现出良好的重现性和选择性，并成功应用于药物制剂、合成尿液和河水水样中氧氟沙星的测定。所提出的方法被证明是优良的；因此，它是测定氧氟沙星的一种替代方法。
