Stevens R W, Elmendorf D, Gourlay M, Stroebel E, Gaafar H A
J Clin Microbiol. 1979 Sep;10(3):346-50. doi: 10.1128/jcm.10.3.346-350.1979.
Special solid-phase fluoroimmunoasssay protocols were used to measure the amount of immunoglobulin G (IgG) and albumin in 1,511 samples of cerebrospinal fluid. The fluoroimmunoassay is an inhibition test conducted on the surface of a plastic probe, whose fluorescence is inversely related to the concentration of spinal fluid IgG or albumin. A microcomputer interfaced with the fluorometer calculates the sample IgG or albumin from a calibration curve based on standard cerebrospinal fluid values. The test and interpretation take less than 90 min. Correlation coefficients for over 100 cerebrospinal fluid samples tested by both fluoroimmunoassay and radial immunodiffusion were: IgG, 0.90 (slope, 1.04); albumin, 0.95 (slope, 0.99). The within-run precision (coefficient of variation) was: IgG, 4.4%; albumin, 6.3%. Run-to-run precision on a midrange sample was: IgG, 7.7%, albumin, 11.7%. These findings establish the simplicity, speed, and precision of the modified fluoroimmunoassay system for specific cerebrospinal fluid proteins.
采用特殊的固相荧光免疫分析方法检测了1511份脑脊液样本中的免疫球蛋白G（IgG）和白蛋白含量。荧光免疫分析是在塑料探针表面进行的抑制试验，其荧光与脑脊液IgG或白蛋白浓度呈负相关。与荧光计相连的微型计算机根据标准脑脊液值通过校准曲线计算样本中的IgG或白蛋白。检测和结果判读耗时不到90分钟。通过荧光免疫分析和放射免疫扩散法检测的100多份脑脊液样本的相关系数分别为：IgG，0.90（斜率，1.04）；白蛋白，0.95（斜率，0.99）。批内精密度（变异系数）分别为：IgG，4.4%；白蛋白，6.3%。中值样本的批间精密度分别为：IgG，7.7%，白蛋白，11.7%。这些结果证实了改良荧光免疫分析系统检测特定脑脊液蛋白质的简便性、快速性和精确性。
