Yang Yunhan, Dong Wenting, Wu Qiuli, Wang Dayong
Medical School, Southeast University Nanjing 210009 China
Nanoscale Adv. 2021 Feb 17;3(7):1997-2006. doi: 10.1039/d0na00867b. eCollection 2021 Apr 6.
The deposition of a certain amount of nanopolystyrene (NPS) can be observed in the gonad of . However, we still know little about the response of germline towards NPS exposure. In the germline of , NPS (1-1000 μg L) increased the expression levels of two G protein-coupled receptors (GPCRs), namely PAQR-2 and CED-1. Moreover, susceptibility to NPS toxicity was observed in worms, which suggested that the protective response of germline was mediated by GPCR CED-1. In the germline, five proteins (CED-10, VPS-34, SNX-1, RAB-7, and RAB-14) functioned as downstream targets of GPCR CED-1 in controlling NPS toxicity. Furthermore, these five targets in the germline regulated NPS toxicity by affecting the activities of p38 MAPK and insulin signaling pathways in intestinal cells. Therefore, we raised a GPCR CED-1-mediated signaling cascade in the germline in response to NPS exposure, which is helpful for understanding the molecular basis of the germline in response to NPS exposure.
在……的性腺中可以观察到一定量的纳米聚苯乙烯（NPS）沉积。然而，我们对生殖系对NPS暴露的反应仍知之甚少。在……的生殖系中，NPS（1 - 1000μg/L）增加了两种G蛋白偶联受体（GPCR），即PAQR - 2和CED - 1的表达水平。此外，在……线虫中观察到对NPS毒性的易感性，这表明生殖系的保护反应是由GPCR CED - 1介导的。在生殖系中，五种蛋白质（CED - 10、VPS - 34、SNX - 1、RAB - 7和RAB - 14）作为GPCR CED - 1在控制NPS毒性方面的下游靶点发挥作用。此外，生殖系中的这五个靶点通过影响肠道细胞中p38 MAPK和胰岛素信号通路的活性来调节NPS毒性。因此，我们提出了生殖系中一种由GPCR CED - 1介导的信号级联反应以应对NPS暴露，这有助于理解生殖系对NPS暴露反应的分子基础。
