Bobade Chandrashekhar D, Nandi Semonti, Kale Narendra R, Banerjee Shashwat S, Patil Yuvraj N, Khandare Jayant J
MAAER's Maharashtra Institute of Pharmacy Kothrud Pune 411038 India.
Maharashtra Institute of Medical Education and Research Medical College Talegaon-Dabhade Pune 410507 India
Nanoscale Adv. 2020 Apr 1;2(6):2315-2325. doi: 10.1039/d0na00075b. eCollection 2020 Jun 17.
Spatial arrangement for cells and the opportunity thereof have implications in cell regeneration and cell proliferation. 3D inverse space (3DIS) substrates with micron-sized pores are fabricated under controlled environmental conditions from polymers such as poly(lactic--glycolic) acid (PLGA), poly(lactic acid) (PLA) and poly(styrene) (PS). The characterization of 3DIS substrates by optical microscopy, scanning probe microscopy (SPM), shows pores within 1-18 μm diameter and prominent surface roughness extending up to 3.9 nm in height over its base. Conversely, to compare two-dimensional (2D) 3DIS substrates, the crucial variables of cell height, cell spreading area and cell volume are compared using lung adenocarcinoma (A549) cells. The results indicate an average cell thickness of ∼6 μm on a glass substrate whereas cells on PLGA 3DIS were ∼12 μm in height, occasionally reaching 20 μm, with a 40% decreased cell spreading area. A549 cells cultured on polymer 3DIS substrates show a cell regeneration growth pattern, dependent on the available spatial volume. Furthermore, PLGA 3DIS cell culture systems with and without graded doxorubicin (DOX) pre-treatment result in potent cell inhibition and cell proliferation, respectively. Additionally, standard DOX administration to A549 cells in the PLGA 3DIS system revealed altered drug sensitivity. 3DIS demonstrates utility in facilitating cellular regeneration and mimicking cell proliferation in defined spatial arrangements.
细胞的空间排列及其机会对细胞再生和细胞增殖具有影响。具有微米级孔隙的3D逆空间（3DIS）基质是在受控环境条件下由聚（乳酸-乙醇酸）（PLGA）、聚乳酸（PLA）和聚苯乙烯（PS）等聚合物制成的。通过光学显微镜、扫描探针显微镜（SPM）对3DIS基质进行表征，结果显示其孔隙直径在1-18μm之间，在其基底上表面粗糙度突出，高度可达3.9nm。相反，为了比较二维（2D）3DIS基质，使用肺腺癌（A549）细胞比较细胞高度、细胞铺展面积和细胞体积等关键变量。结果表明，在玻璃基质上细胞的平均厚度约为6μm，而在PLGA 3DIS上的细胞高度约为12μm，偶尔可达20μm，细胞铺展面积减少了40%。在聚合物3DIS基质上培养的A549细胞呈现出细胞再生生长模式，这取决于可用的空间体积。此外，分别经过分级阿霉素（DOX）预处理和未经过预处理的PLGA 3DIS细胞培养系统，前者导致有效的细胞抑制，后者导致细胞增殖。此外，在PLGA 3DIS系统中对A549细胞进行标准的DOX给药显示出药物敏感性的改变。3DIS在促进细胞再生和模拟特定空间排列中的细胞增殖方面显示出实用性。
