Siopi Maria, Papadopoulos Antonios, Spiliopoulou Anastasia, Paliogianni Fotini, Abou-Chakra Nissrine, Arendrup Maiken Cavling, Damoulari Christina, Tsioulos Georgios, Giannitsioti Efthymia, Frantzeskaki Frantzeska, Tsangaris Iraklis, Pournaras Spyros, Meletiadis Joseph
Clinical Microbiology Laboratory, Attikon University General Hospital, Medical School, National and Kapodistrian University of Athens, 12462 Athens, Greece.
4th Department of Internal Medicine, Attikon University General Hospital, Medical School, National and Kapodistrian University of Athens, 12462 Athens, Greece.
J Fungi (Basel). 2022 Sep 1;8(9):931. doi: 10.3390/jof8090931.
The isolation of a pan-echinocandin-resistant Candida parapsilosis strain (anidulafungin, caspofungin, micafungin and rezafungin EUCAST MICs > 8 mg/L) from urine of a patient following prolonged exposure to echinocandins (38 days of micafungin followed by 16 days of anidulafungin) is described. The isolate harbored the novel alteration F652S in the hotspot 1 region of fks1. Isogenic C. parapsilosis bloodstream isolates collected up to 1.5 months earlier from the same patient were susceptible to echinocandins (anidulafungin, caspofungin and micafungin EUCAST MICs 1−2, 1 and 1 mg/L, respectively) and contained wild-type FKS1 sequences. This is the first report of pan-echinocandin resistance in C. parapsilosis associated with an aminoacid change in hotspot 1 region of fks1.
本文描述了从一名长期接受棘白菌素治疗（先使用米卡芬净38天，后使用阿尼芬净16天）的患者尿液中分离出一株对所有棘白菌素耐药的近平滑念珠菌菌株（阿尼芬净、卡泊芬净、米卡芬净和瑞扎芬净的欧洲抗微生物药敏试验委员会（EUCAST）最低抑菌浓度（MIC）>8 mg/L）。该分离株在fks1热点1区域存在新型突变F652S。在更早之前（最早提前1.5个月）从同一患者收集的近平滑念珠菌血流分离株对棘白菌素敏感（阿尼芬净、卡泊芬净和米卡芬净的EUCAST MIC分别为1−2、1和1 mg/L），且含有野生型FKS1序列。这是首例与fks1热点1区域氨基酸变化相关的近平滑念珠菌对所有棘白菌素耐药的报道。
