Institute of Genetics and Biotechnology, Department of Animal Biotechnology, Hungarian University of Agriculture and Life Sciences, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Szent-Györgyi Albert Street 4, H-2100 Gödöllő, Hungary.
Institute of Physiology and Nutrition, Department of Feed Safety, Hungarian University of Agriculture and Life Sciences, Páter K. Street 1, H-2100 Gödöllő, Hungary.
Toxins (Basel). 2022 Sep 13;14(9):634. doi: 10.3390/toxins14090634.
Mycotoxins are bioaccumulative contaminants impacting animals and humans. The simultaneous detection of frequent active exposures and accumulated mycotoxin level (s) in exposed organisms would be the most ideal to enable appropriate actions. However, few methods are available for the purpose, and there is a demand for dedicated, sensitive, reliable, and practical assays. To demonstrate the issue, mice were exposed to a relevant agent Ochratoxin A (OTA), and accumulated OTA was measured by fine-tuned commercial assays. Quantitative high-performance liquid chromatography with fluorescence detection, enzyme-linked immunosorbent assay, and flow cytometry assays have been developed/modified using reagents available as commercial products when appropriate. Assays were performed on excised samples, and results were compared. Accumulated OTA could be detected and quantified; positive correlations (between applied doses of exposure and accumulated OTA levels and the results from assays) were found. Dedicated assays could be developed, which provided comparable results. The presence and accumulation of OTA following even a short exposure could be quantitatively detected. The assays performed similarly, but HPLC had the greatest sensitivity. Blood contained higher levels of OTA than liver and kidney. We demonstrate that specific but flexible and practical assays should be used for specific/local purposes, to measure the exposure itself and accumulation in blood or organs.
真菌毒素是具有生物累积性的污染物，会对动物和人类造成影响。在暴露的生物体中同时检测频繁的主动暴露和累积的真菌毒素水平（s）将是最理想的，以便能够采取适当的措施。然而，为此目的可用的方法很少，并且需要专用的、敏感的、可靠的和实用的检测方法。为了说明这个问题，我们用相关试剂 Ochratoxin A (OTA) 使小鼠暴露，并使用微调的商业检测方法来测量累积的 OTA。已经使用适当的商业产品中的试剂开发/修改了定量高效液相色谱法与荧光检测、酶联免疫吸附测定和流式细胞术检测方法。在切除的样本上进行了检测，并比较了结果。可以检测和定量累积的 OTA；发现了（暴露应用剂量与累积 OTA 水平之间的）阳性相关性（和检测结果之间的相关性）。可以开发专用的检测方法，这些方法提供了可比的结果。即使是短暂的暴露，也可以定量检测到 OTA 的存在和积累。尽管检测方法类似，但 HPLC 的灵敏度最高。血液中 OTA 的含量高于肝脏和肾脏。我们证明，应该根据具体的局部目的使用特定的、灵活的和实用的检测方法，来测量暴露本身和血液或器官中的积累情况。
