希腊屠宰猪血清中赭曲霉毒素 A 残留的存在。
Presence of Ochratoxin A Residues in Blood Serum of Slaughtered Pigs in Greece.
机构信息
Laboratory of Hygiene of Foods of Animal Origin, Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.
Department of Food Science and Nutrition, University of Thessaly, Terma N. Temponera Street, 43100 Karditsa, Greece.
出版信息
Toxins (Basel). 2024 Sep 30;16(10):421. doi: 10.3390/toxins16100421.
This study aimed to assess the presence of ochratoxin A (OTA) residues in the blood serum of slaughtered pigs in Greece. Samples were obtained from 1695 healthy slaughtered pigs originating from 113 different farms located in 21 geographic regional units in 8 different geographic regions of Greece and were analyzed using an immunosorbent assay (ELISA) and high-performance liquid chromatography with fluorescence detector (HPLC-FD). OTA contamination assessment showed that 782 (46.1%) and 1233 (72.7%) samples were OTA-positive, with a concentration range of 0.20-5.38 μg/L and 0.15-5.96 µg/L according to ELISA and HPLC-FD analysis, respectively. Also, 88 (77.9%) and 108 (95.6%) of farms were found to be OTA-positive by ELISA and HPLC-FD analysis, respectively. The highest OTA serum positivity rate (>98%) and toxin level (5.96 µg/L) determined by HPLC-FD were observed in the Thessaly region, whereas a high prevalence of up to 100% (range 75-100%) was found on farms in the Crete Island region. The detection of OTA in the serum of slaughtered pigs in different regions in Greece poses a risk for animal and human health and highlights the need for constant OTA monitoring in the swine industry and pork meat production facilities.
本研究旨在评估希腊屠宰猪血清中赭曲霉毒素 A（OTA）残留情况。从希腊 8 个不同地区的 21 个地理区域单位的 113 个不同农场的 1695 头健康屠宰猪中采集样本，使用免疫吸附测定（ELISA）和高效液相色谱-荧光检测（HPLC-FD）进行分析。OTA 污染评估显示，782（46.1%）和 1233（72.7%）份样品呈 OTA 阳性，ELISA 和 HPLC-FD 分析的浓度范围分别为 0.20-5.38 μg/L 和 0.15-5.96 μg/L。此外，ELISA 和 HPLC-FD 分析分别发现 88（77.9%）和 108（95.6%）个农场呈 OTA 阳性。通过 HPLC-FD 检测到的 OTA 血清阳性率最高（>98%）和毒素水平（5.96 μg/L）出现在色萨利地区，而克里特岛地区的农场阳性率高达 100%（75-100%）。希腊不同地区屠宰猪血清中 OTA 的检测对动物和人类健康构成威胁，这突显了在养猪业和猪肉生产设施中持续监测 OTA 的必要性。
Zotero 插件