Department of Pharmacy, Lishui Hospital of Traditional Chinese Medicine, Lishui, Zhejiang, China.
School of Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing, China.
PLoS One. 2022 Sep 22;17(9):e0274358. doi: 10.1371/journal.pone.0274358. eCollection 2022.
The dried plant material of medically important plant Actinidia eriantha especially when it remains in the form of powder often look morphologically similar to its related species. The lack of efficient methods to distinguish the authentic material from other similar species leads to chances of adulteration. The molecular authentication of herbal plant materials such as the internal transcribed spacer (ITS) sequences is considered as more reliable method compared to morphological traits. In this study, we aim to evaluate the potential of identification for roots of A. eriantha and its related species by ITS sequences. The lengths of ITS regions ranged from 624 to 636 bp with GC content ranging from 50.96% to 59.55%. A total of 194 variation sites and 46 haplotypes were formed in 185 samples. Among them, the roots of A. eriantha possessed specific sites at 85bp (C), 205bp (T), 493bp (C), 542bp (G), 574bp (C), 582bp (T) and 610bp (G), while A. hemsleyana, A. callosa, A. valvata and A. polygama have their own specific sites. The inter-specific genetic distance among 8 Actinidia species in the range 2.28% to 11.00%. The phylogenetic tree constructed with ITS, ITS1 and ITS2 region showed that the ITS sequences have higher potential for identification in 8 Actinidia species. However, as to A. eriantha, A. hemsleyana and A. valvata, these three barcodes have the same identification ability. The ITS regions indicated that different samples from same species can be grouped together, except for A. arguta and A. melanandrah. In conclusion, the ITS sequences can be used as an efficient DNA barcode for the identification of A. eriantha and its related species.
药用植物猕猴桃的干植物材料，特别是当它保持粉末形式时，通常在形态上与相关物种相似。缺乏有效区分正品材料与其他类似物种的方法会导致掺假的机会。与形态特征相比，内部转录间隔区（ITS）序列等草药植物材料的分子鉴定被认为是更可靠的方法。在这项研究中，我们旨在通过 ITS 序列评估鉴定猕猴桃及其相关物种根的潜力。ITS 区域的长度范围为 624 至 636 bp，GC 含量范围为 50.96%至 59.55%。在 185 个样本中形成了总共 194 个变异位点和 46 个单倍型。其中，猕猴桃的根在 85bp（C）、205bp（T）、493bp（C）、542bp（G）、574bp（C）、582bp（T）和 610bp（G）处具有特定位点，而中华猕猴桃、软枣猕猴桃、狗枣猕猴桃和葛枣猕猴桃则具有各自的特定位点。8 种猕猴桃属植物的种间遗传距离在 2.28%至 11.00%之间。基于 ITS、ITS1 和 ITS2 区构建的系统发育树表明，ITS 序列在 8 种猕猴桃属植物的鉴定中具有更高的潜力。然而，对于猕猴桃、中华猕猴桃和狗枣猕猴桃，这三个条形码具有相同的鉴定能力。ITS 区表明，来自同一物种的不同样本可以聚在一起，除了软枣猕猴桃和毛花猕猴桃。总之，ITS 序列可用于鉴定猕猴桃及其相关物种。
