Chen Lijuan, Zhang Zhongjie, Chen Kai, Yu Ye, Hu Bo, Song Hongsheng, Liu Xiaojing
College of Life Sciences, Shanghai University, Shanghai 200444, China.
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
Biology (Basel). 2022 Sep 4;11(9):1313. doi: 10.3390/biology11091313.
Diapause is a form of dormancy that organisms use to adapt to extreme environments by exhibiting developmental arrest. In the silkworm, , diapause is thought to be elicited by diapause hormone (DH) signaling, which consists of interactions between DH and the DH receptor (DHR). However, the steps downstream of the DH signaling pathway are largely unknown. In the present study, we directly injected synthesized DH into the female pupae of a multivoltine, non-diapausing strain at 36 h after pupation. We found that the mRNA level of declined at 4 h and recovered at 12 h after the injection of DH. Thus, we sequenced the transcriptome of the ovaries at 4 h and 12 h after the injection of DH. We identified 60 and 221 differentially expressed genes at 4 h and 12 h after the injection, respectively. All DEGs were identified, relating to 20E-related genes, JH-related genes, cellular detoxification, ribosomal proteins, lipid metabolism, and epigenetic modifications. Eleven genes were selected from the above categories to verify the transcriptome data. The qRT-PCR and RNA-Seq expression patterns of the genes were consistent, which indicated the authenticity and reliability of the transcriptome data. This study dramatically expands upon our knowledge of gene expression variation at the early phase of DH release.
滞育是生物体用来通过表现出发育停滞来适应极端环境的一种休眠形式。在家蚕中，滞育被认为是由滞育激素（DH）信号引发的，该信号由DH与DH受体（DHR）之间的相互作用组成。然而，DH信号通路下游的步骤在很大程度上尚不清楚。在本研究中，我们在化蛹后36小时将合成的DH直接注射到一个多化性、非滞育品系的雌蛹中。我们发现，注射DH后4小时， 的mRNA水平下降，并在12小时后恢复。因此，我们对注射DH后4小时和12小时的卵巢转录组进行了测序。我们分别在注射后4小时和12小时鉴定出60个和221个差异表达基因。所有差异表达基因均被鉴定出来，涉及与20E相关的基因、与JH相关的基因、细胞解毒、核糖体蛋白、脂质代谢和表观遗传修饰。从上述类别中选择了11个基因来验证转录组数据。这些基因的qRT-PCR和RNA-Seq表达模式一致，这表明转录组数据的真实性和可靠性。这项研究极大地扩展了我们对DH释放早期基因表达变化的认识。
