Zhang Xue, Du Wenmei, Zhang Junjie, Zou Zhen, Ruan Changchun
Engineering Research Center of Natural Enemies, Institute of Biological Control, Jilin Agricultural University, Changchun, 130118, China.
State Key Laboratory of Integrated Management of Pest Insect and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
BMC Genomics. 2020 Dec 4;21(1):864. doi: 10.1186/s12864-020-07285-4.
The parasitoid wasp, Trichogramma dendrolimi, can enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi. Previous studies on diapause have mainly focused on ecological characteristics, so the molecular basis of diapause in T. dendrolimi is unknown. We compared transcriptomes of diapause and non-diapause T. dendrolimi to identify key genes and pathways involved in diapause development.
Transcriptome sequencing was performed on diapause prepupae, pupae after diapause, non-diapause prepupae, and pupae. Analysis yielded a total of 87,022 transcripts with an average length of 1604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7593 sequences with an average length of 3351 bp. Among them, 5702 genes were differentially expressed. The result of Gene Ontology (GO) enrichment analysis revealed that regulation of transcription, DNA-templated, oxidation-reduction process, and signal transduction were significantly affected. Ten genes were selected for validation using quantitative real-time PCR (qPCR). The changes showed the same trend as between the qPCR and RNA-Seq results. Several genes were identified as involved in diapause, including ribosomal proteins, zinc finger proteins, homeobox proteins, forkhead box proteins, UDP-glucuronosyltransferase, Glutathione-S-transferase, p53, and DNA damage-regulated gene 1 (pdrg1). Genes related to lipid metabolism were also included.
We generated a large amount of transcriptome data from T. dendrolimi, providing a resource for future gene function research. The diapause-related genes identified help reveal the molecular mechanisms of diapause, in T. dendrolimi, and other insect species.
拟澳洲赤眼蜂（Trichogramma dendrolimi）这种寄生蜂在蛹前期可进入滞育状态。因此，滞育是拟澳洲赤眼蜂大规模生产过程中的一种有效保存方法。以往关于滞育的研究主要集中在生态特征方面，所以拟澳洲赤眼蜂滞育的分子基础尚不清楚。我们比较了滞育和非滞育拟澳洲赤眼蜂的转录组，以确定参与滞育发育的关键基因和途径。
对滞育蛹前期、滞育后蛹、非滞育蛹前期和蛹进行了转录组测序。分析共产生了87,022条转录本，平均长度为1604 bp。通过去除冗余序列和那些无显著BLAST匹配的序列，生成了一个非冗余数据集，包含7593条序列，平均长度为3351 bp。其中，5702个基因差异表达。基因本体（GO）富集分析结果显示，DNA模板转录调控、氧化还原过程和信号转导受到显著影响。选择了10个基因使用定量实时PCR（qPCR）进行验证。结果变化趋势与qPCR和RNA-Seq结果一致。鉴定出几个与滞育相关的基因，包括核糖体蛋白、锌指蛋白、同源框蛋白、叉头框蛋白、尿苷二磷酸葡萄糖醛酸基转移酶、谷胱甘肽-S-转移酶、p53和DNA损伤调节基因1（pdrg1）。还包括与脂质代谢相关的基因。
我们从拟澳洲赤眼蜂中生成了大量转录组数据，为未来的基因功能研究提供了资源。鉴定出的滞育相关基因有助于揭示拟澳洲赤眼蜂以及其他昆虫物种滞育的分子机制。
