Morandi Anita, Corradi Massimiliano, Zusi Chiara, Piona Claudia, Costantini Silvia, Marigliano Marco, Maffeis Claudio
Section of Pediatric Diabetes and Metabolism, Department of Surgery, Dentistry, Pediatrics, and Gynecology, University of Verona, Piazzale Stefani, 37126 Verona, Italy.
Antioxidants (Basel). 2022 Aug 31;11(9):1726. doi: 10.3390/antiox11091726.
The minor allele of the single nucleotide polymorphism (SNP) rs2364723 of , a gene encoding a master antioxidant transcription factor, has been associated with poor cardiovascular outcomes and with complications of type 2 diabetes. We assessed the association between rs2364723 of and oxidative stress in children/adolescents with type 1 diabetes (T1D).
In 384 children/adolescents with T1D (age 15.7 ± 3.2 years, 207 males), we assessed the oxidative stress by measuring the concentration of derivatives of reactive oxygen metabolites (d-ROMs) and we genotyped the rs2364723 SNP by real time polymerase chain reaction.
The concentration of d-ROMs was 372.8 ± 64.6 Carratelli units. The minor genotype (CC) of rs2364723 at was associated with higher concentration of derivatives of d-ROMs in the subgroup with HbA1c ≥ 8% (B = 47.85, p for genotype ∗ HbA1c interaction = 0.019).
The carriers of the minor genotype of rs2364723 may have increased oxidative stress compared to their counterparts with other genotypes, especially in case of poor glycemic control. This observation needs to be replicated and confirmed in larger independent cohorts of youth with T1D.
编码主要抗氧化转录因子的基因的单核苷酸多态性（SNP）rs2364723的次要等位基因与不良心血管结局以及2型糖尿病并发症相关。我们评估了1型糖尿病（T1D）儿童/青少年中rs2364723与氧化应激之间的关联。
在384例T1D儿童/青少年（年龄15.7±3.2岁，男性207例）中，我们通过测量活性氧代谢产物衍生物（d-ROMs）的浓度来评估氧化应激，并通过实时聚合酶链反应对rs2364723 SNP进行基因分型。
d-ROMs的浓度为372.8±64.6卡拉泰利单位。在HbA1c≥8%的亚组中，rs2364723的次要基因型（CC）与d-ROMs衍生物的较高浓度相关（B = 47.85，基因型*HbA1c相互作用的p值= 0.019）。
与其他基因型的对应者相比，rs2364723次要基因型的携带者可能具有更高的氧化应激，尤其是在血糖控制不佳的情况下。这一观察结果需要在更大的T1D青年独立队列中重复和证实。
