Oikonomidis Ioannis L, Kiosis Evangelos A, Brozos Christos N, Kritsepi-Konstantinou Maria G
Am J Vet Res. 2017 Mar;78(3):274-278. doi: 10.2460/ajvr.78.3.274.
OBJECTIVE To establish reference intervals for serum reactive oxygen metabolites (ROMs), biological antioxidant potential (BAP), and oxidative stress index (OSi) in adult rams by use of controlled preanalytic and analytic procedures. ANIMALS 123 healthy 1- to 4-year-old rams of 2 Greek breeds (Chios [n = 62] and Florina [61]). PROCEDURES 4 hours after rams were fed, a blood sample was obtained from each ram, and serum was harvested. Concentrations of ROMs and BAP were measured colorimetrically on a spectrophotometric analyzer. The OSi was calculated as ROMs concentration divided by BAP concentration. Combined and breed-specific reference intervals were calculated by use of nonparametric and robust methods, respectively. Reference intervals were defined as the 2.5th to 97.5th percentiles. RESULTS Reference intervals for ROMs, BAP, and OSi for all rams combined were 65 to 109 Carratelli units, 2,364 to 4,491 μmol/L, and 18.2 to 43.0 Carratelli units/(mmol/L), respectively. Reference intervals of Chios rams for ROMs, BAP, and OSi were 56 to 113 Carratelli units, 2,234 to 4,290 μmol/L, and 12.9 to 38.4 Carratelli units/(mmol/L), respectively. Reference intervals of Florina rams for ROMs, BAP, and OSi were 68 to 111 Carratelli units, 2,337 to 4,363 μmol/L, and 14.1 to 38.1 Carratelli units/(mmol/L), respectively. CONCLUSIONS AND CLINICAL RELEVANCE Reference intervals calculated in this study can be used as a guide for the interpretation of ROMs, BAP, and OSi results in rams and, under appropriate conditions, can be adopted for use by veterinary laboratories.
目的 通过采用可控的分析前和分析程序，建立成年公羊血清活性氧代谢产物（ROMs）、生物抗氧化潜能（BAP）和氧化应激指数（OSi）的参考区间。动物 123 只健康的 1 至 4 岁希腊两个品种（希俄斯岛羊[n = 62]和弗洛里纳羊[61]）的公羊。程序 在公羊进食后 4 小时，从每只公羊采集血样并收获血清。在分光光度计分析仪上用比色法测量 ROMs 和 BAP 的浓度。OSi 计算为 ROMs 浓度除以 BAP 浓度。分别使用非参数和稳健方法计算合并的和特定品种的参考区间。参考区间定义为第 2.5 至 97.5 百分位数。结果 所有公羊合并的 ROMs、BAP 和 OSi 的参考区间分别为 65 至 109 卡拉泰利单位、2364 至 4491 μmol/L 和 18.2 至 43.0 卡拉泰利单位/（mmol/L）。希俄斯岛羊的 ROMs、BAP 和 OSi 的参考区间分别为 56 至 113 卡拉泰利单位、2234 至 4290 μmol/L 和 12.9 至 38.4 卡拉泰利单位/（mmol/L）。弗洛里纳羊的 ROMs、BAP 和 OSi 的参考区间分别为 68 至 111 卡拉泰利单位、2337 至 4363 μmol/L 和 14.1 至 38.1 卡拉泰利单位/（mmol/L）。结论及临床意义 本研究计算的参考区间可作为公羊 ROMs、BAP 和 OSi 结果解释的指南，并在适当条件下可供兽医实验室采用。
