Khan Zaheer Ullah, Khan Taous, Mannan Abdul, Ali Atif, Ni Jiang
Department of Pharmacy, Abbottabad Campus, COMSATS University Islamabad, Abbottabad 22010, Pakistan.
Department of Pharmacy, Affiliated Hospital of Jiangnan University, Wuxi 214000, China.
Biomedicines. 2022 Sep 13;10(9):2266. doi: 10.3390/biomedicines10092266.
Although L. extract (M-Ext) of the peel and kernel possesses potent antioxidant and excellent antiaging qualities, the effects are only partially seen because of the skin's limited ability to absorb it. M-Ext was loaded into nanolipid carriers (M-NLCs) in this work to create a green topical formulation that would boost antiaging efficacy and address penetration deficit. Compound identification was done using GCMS and atomic absorption spectroscopy for heavy metals in M-Ext. M-Ext was also evaluated against oxidative stress antioxidant enzymes. The M-NLCs were fabricated and evaluated for their physicochemical characterizations. Cytotoxicity and cell permeation analysis of M-Ext and M-NLCs were carried out in fibroblasts and HaCaT cell lines. An ex vivo permeation study of M-Ext and M-NLC-loaded emulsion was performed through rat skin and the kinetic parameters were determined. Kinetic data showed that the ex vivo permeation of M-NLC-loaded emulsion through rat skin followed the Higuchi model. The safety profile was evaluated in human volunteers after written consent. Three months' in vivo investigations were conducted using the optimized M-NLC-loaded emulsion and vehicle (B-NLC-loaded emulsion) on human cheeks for comparison. The volunteers' skin erythema level, melanin contents, TEWL index, moisture contents, sebum level, elasticity, pH, and pore size were examined after the first, second, and third month via noninvasive techniques. There were significant findings for physicochemical characterizations and in vitro and ex vivo studies. The findings demonstrate that the green nanolipid carriers amplified the overall antioxidant effectiveness and may represent an emerging treatment strategy for oxidative stresses and aging.
尽管果皮和果仁的L.提取物（M-Ext）具有强大的抗氧化和出色的抗老化特性，但由于皮肤吸收能力有限，这些效果只能部分显现。在这项研究中，M-Ext被载入纳米脂质载体（M-NLCs），以创建一种绿色外用制剂，提高抗老化功效并解决渗透不足的问题。使用气相色谱-质谱联用仪（GCMS）和原子吸收光谱法对M-Ext中的重金属进行化合物鉴定。还对M-Ext进行了针对氧化应激抗氧化酶的评估。制备了M-NLCs并对其进行了理化性质表征。在成纤维细胞和HaCaT细胞系中对M-Ext和M-NLCs进行了细胞毒性和细胞渗透分析。通过大鼠皮肤对M-Ext和载有M-NLC的乳液进行了体外渗透研究，并确定了动力学参数。动力学数据表明，载有M-NLC的乳液通过大鼠皮肤的体外渗透遵循Higuchi模型。在获得书面同意后，对人类志愿者进行了安全性评估。使用优化的载有M-NLC的乳液和赋形剂（载有B-NLC的乳液）在人类脸颊上进行了为期三个月的体内研究以作比较。在第一个月、第二个月和第三个月后，通过非侵入性技术检查了志愿者的皮肤红斑水平、黑色素含量、经皮水分流失（TEWL）指数、水分含量、皮脂水平、弹性、pH值和孔径。在理化性质表征以及体外和体内研究方面有重要发现。这些发现表明，绿色纳米脂质载体增强了整体抗氧化效果，可能代表了一种针对氧化应激和衰老的新兴治疗策略。
