Waqas Muhammad Khurram, Khan Barkat Ali, Akhtar Naveed, Chowdhry Farzana, Khan Haroon, Bakhsh Sattar, Khan Shahzeb, Rasul Akhtar
Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Faculty of Pharmacy, Gomal University D.I Khan, Dera Ismail Khan, Pakistan.
Postepy Dermatol Alergol. 2017 Aug;34(4):339-345. doi: 10.5114/ada.2017.69314. Epub 2017 Aug 1.
Intracellular and extracellular oxidative stress triggered by free radicals promotes skin aging, which is designated by atypical pigmentation and wrinkles. The consumption of antioxidants is an efficacious measure to avert symptoms involved in skin aging.
The current research was commenced to explore the anti-aging potential of antioxidants present in seeds extract.
seeds extract was obtained by concentrating the ethanolic extract of seeds. The antioxidant activities of the extract were measured by nitric oxide radical scavenging assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, hydroxyl radical scavenging assay and superoxide radical scavenging assay. Formulation comprising 4% of the concentrated extract of seeds was formulated by loading it in the internal aqueous phase of water-in-oil (W/O) cosmetic emulsion. The base, used as control, consisted of the same emulsion but without loading seeds extract. The cosmetic emulsions were applied to the cheeks of 11 healthy male volunteers for duration of 12 weeks. Both base and formulation were assessed for their antioxidant effects on different skin parameters i.e. skin moisture contents, elasticity and surface evaluation of living skin (SELS).
The formulation showed statistically significant ( ≤ 0.05) and the base showed insignificant ( > 0.05) effects on skin elasticity and skin moisture contents. There is a significant decline in SELS, skin scaliness (SEsc), skin wrinkles (SEw), skin smoothness (SEsm), and skin roughness (SEr) parameters after application of the formulation.
Topical application of the cosmetic emulsion entrapped with seeds extract containing various antioxidants exerts potential skin antiaging effects.
自由基引发的细胞内和细胞外氧化应激会促进皮肤衰老，表现为非典型色素沉着和皱纹。食用抗氧化剂是预防皮肤衰老相关症状的有效措施。
开展本研究以探索种子提取物中抗氧化剂的抗衰老潜力。
通过浓缩种子的乙醇提取物获得种子提取物。采用一氧化氮自由基清除试验、2,2-二苯基-1-苦基肼（DPPH）自由基清除试验、羟基自由基清除试验和超氧阴离子自由基清除试验测定提取物的抗氧化活性。将含有4%种子浓缩提取物的制剂通过负载于油包水（W/O）型化妆品乳液的内水相中进行配制。用作对照的基质由相同的乳液组成，但未负载种子提取物。将化妆品乳液应用于11名健康男性志愿者的脸颊，持续12周。评估基质和制剂对不同皮肤参数的抗氧化作用，即皮肤水分含量、弹性和活体皮肤表面评估（SELS）。
制剂对皮肤弹性和皮肤水分含量有统计学显著影响（≤0.05），而基质无显著影响（>0.05）。应用制剂后，SELS、皮肤鳞屑（SEsc）、皮肤皱纹（SEw）、皮肤光滑度（SEsm）和皮肤粗糙度（SEr）参数有显著下降。
局部应用含有多种抗氧化剂的包裹种子提取物的化妆品乳液具有潜在的皮肤抗衰老作用。
