Laboratoire de Recherche en Production et Santé Animales (LaRePSA), Institut de l'Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso.
Institut du Développement Rural, Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina Faso.
Genes (Basel). 2022 Aug 24;13(9):1523. doi: 10.3390/genes13091523.
The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogenetic relationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (F) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (F) and among-individual differentiation (F), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso.
本研究旨在利用微卫星标记调查布基纳法索地方鸡生态型的遗传多样性和群体结构。使用 20 个选定的多态微卫星标记，共 71 只代表中东部（18 只）、中北部（17 只）、萨赫勒（18 只）和西南部（18 只）地方鸡群体的个体，估算遗传多样性指数、群体结构和系统发育关系。等位基因数、平均等位基因数、观察和预期杂合度均值、多态信息含量分别为 127、6.35、0.391、0.521、0.539 和 0.541。估计的群体间固定指数（F）、群体间（F）和鸡生态型内近交系数分别为 0.239、0.267 和 0.243。分子方差分析表明，总遗传多样性的 77%归因于群体内变异，其余 1%和 22%归因于区域间分化（F）和个体间分化（F）。地方 Konde 生态型与中北部地区的鸡之间的遗传距离最大（0.026），而萨赫勒和中北部地区的鸡之间的遗传距离最小（0.003）。邻接聚类树和主成分判别分析证实了群体间观察到的遗传距离。结果表明，布基纳法索的地方鸡具有丰富的遗传多样性，研究群体之间的分化程度较小。本研究提供了有关遗传多样性衡量标准的重要信息，有助于未来为布基纳法索的地方鸡设计和实施遗传改良和保护计划。
