Institute of Anatomy, University Medicine Rostock, University of Rostock, 18051 Rostock, Germany.
Anatomy and Cell Biology, Medical School OWL, Bielefeld University, 33615 Bielefeld, Germany.
Int J Mol Sci. 2022 Sep 6;23(18):10247. doi: 10.3390/ijms231810247.
Microglia play important roles during physiological and pathological situations in the CNS. Several reports have described the expression of in disease-associated and aged microglia. Here, we demonstrated that TGFβ1 controled the expression of in microglia in vitro and in vivo. Using BV2 cells, primary microglia cultures as well as in combination with qPCR, flow cytometry, and immunohistochemistry, we were able to provide evidence that TGFβ1 inhibited LPS-induced upregulation of in microglia. Interestingly, TGFβ1 alone was able to mediate downregulation of CD74 in vitro. Moreover, silencing of TGFβ signaling in vivo resulted in marked upregulation of CD74, further underlining the importance of microglial TGFβ signaling during regulation of microglia activation. Taken together, our data indicated that CD74 is a marker for activated microglia and further demonstrated that microglial TGFβ signaling is important for regulation of expression during microglia activation.
小胶质细胞在中枢神经系统的生理和病理情况下发挥重要作用。有几项报道描述了在与疾病相关和衰老的小胶质细胞中 的表达。在这里，我们证明 TGFβ1 可以控制体外和体内小胶质细胞中 的表达。使用 BV2 细胞、原代小胶质细胞培养物以及 qPCR、流式细胞术和免疫组织化学，我们能够提供证据表明 TGFβ1 抑制 LPS 诱导的小胶质细胞中 的上调。有趣的是，TGFβ1 本身能够在体外介导 CD74 的下调。此外，体内 TGFβ 信号转导的沉默导致 CD74 的明显上调，进一步强调了小胶质细胞 TGFβ 信号在调节小胶质细胞激活中的重要性。总之，我们的数据表明 CD74 是激活的小胶质细胞的标志物，并进一步证明小胶质细胞 TGFβ 信号在小胶质细胞激活过程中调节 的表达是重要的。
