Mycotoxin contamination of maize kernels by fungal pathogens like and is a chronic global challenge impacting food and feed security, health, and trade. Maize genes () synthetize oxylipins that play defense roles and govern host-fungal interactions. The current study investigated the involvement of in maize resistance against these two fungi. A considerable intraspecific genetic and transcript variability of the ZmLOX family was highlighted by in silico analysis comparing publicly available maize pan-genomes and pan-transcriptomes, respectively. Then, phenotyping and expression analysis of genes along with key genes involved in oxylipin biosynthesis were carried out in a maize mutant carrying a Mu transposon insertion in the gene (named UFMu) together with Tzi18, Mo17, and W22 inbred lines at 3- and 7-days post-inoculation with and . Tzi18 showed the highest resistance to the pathogens coupled with the lowest mycotoxin accumulation, while UFMu was highly susceptible to both pathogens with the most elevated mycotoxin content. inoculation determined a stronger induction of and maize genes as compared to . Additionally, oxylipin analysis revealed prevalent linoleic (18:2) peroxidation by 9-LOXs, the accumulation of 10-oxo-11-phytoenoic acid (10-OPEA), and triglyceride peroxidation only in inoculated kernels of resistant genotypes.