2018 年中国由基因 Ib 型日本脑炎病毒引起的日本脑炎暴发：实验室和现场调查。

An outbreak of Japanese encephalitis caused by genotype Ib Japanese encephalitis virus in China, 2018: A laboratory and field investigation.

机构信息

Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, People's Republic of China.

Department of Arbovirus, NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

出版信息

PLoS Negl Trop Dis. 2020 May 26;14(5):e0008312. doi: 10.1371/journal.pntd.0008312. eCollection 2020 May.

DOI:10.1371/journal.pntd.0008312

PMID:32453787

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7274457/

Abstract

Although Japanese encephalitis virus genotype Ib (JEV GIb) has replaced JEV GIII as the dominant genotype in endemic areas of Asia, no JEV GIb has been isolated from JE cases and natural mosquitoes at the same time in an outbreak of JE. In this study, we conducted virological and molecular biological laboratory tests on JE case samples (serum/cerebrospinal fluid) and locally collected mosquito samples from the 2018 JE outbreak in Ningxia, China. The result of JEV IgM antibody detection showed that 96% (67/70) of the suspected cases were laboratory-confirmed JE cases. Of the mosquitoes collected from local environments, 70% (17400/24900) were Culex tritaeniorhynchus of which 4.6% (16 /348 of the pools tested) were positive for JEV, other mosquitoes were negative. JEVs isolated from both the human cases and C. tritaeniorhynchus specimens belong to JEV GIb and are in the same evolutionary clade according to molecular evolution analyses. JEV GIb was detected simultaneously from specimens of JE cases and mosquito samples collected in nature in this study, suggesting that the JE outbreak that occurred in Ningxia in 2018 was due to infection of JEV GIb.

摘要

虽然乙型日本脑炎病毒（JEV GIb）已取代 JEV GIII 成为亚洲流行地区的主要基因型，但在 JEV 暴发疫情中，尚未同时从 JEV 病例和自然蚊虫中分离到 JEV GIb。在这项研究中，我们对来自中国宁夏 2018 年 JEV 暴发的 JEV 病例样本（血清/脑脊液）和当地采集的蚊虫样本进行了病毒学和分子生物学实验室检测。JEV IgM 抗体检测结果显示，96%（67/70）的疑似病例为实验室确诊的 JEV 病例。从当地环境中采集的蚊虫中，70%（17400/24900）为三带喙库蚊，其中 4.6%（16/348 个测试的蚊群）为 JEV 阳性，其他蚊虫均为阴性。从人类病例和 C. tritaeniorhynchus 标本中分离出的 JEV 均属于 JEV GIb，根据分子进化分析，它们属于同一进化枝。本研究同时从 JEV 病例标本和自然采集的蚊虫标本中检测到 JEV GIb，提示 2018 年宁夏发生的 JEV 暴发是由 JEV GIb 感染引起的。