Yadav Pragya, Shete Anita, Bondre Vijay, Patil Deepak, Kokate Prasad, Chaudhari Sweena, Srivastava Sneha, Jadhav Santosh, Mourya Devendra
National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
Infect Genet Evol. 2016 Oct;44:122-126. doi: 10.1016/j.meegid.2016.06.049. Epub 2016 Jun 29.
During a study on Japanese encephalitis (JE) from Kolar district of Karnataka state, India in 1986; two virus isolates were obtained in infant Swiss albino mouse from a pig and a human serum sample. For characterization of these virus isolates, they were propagated in Vero CCL-81 cells. These virus isolates were screened for flaviviruses (Japanese encephalitis, West Nile, Dengue, Kyasanur forest disease) and Alphavirus (Chikungunya) by RT-PCR and found to be negative. Further these they were screened for bunyaviruses using genus-specific primers. A virus isolate from a human sample was sequenced using next generation sequencing; which identified it as Oya virus, Simbu group of the genus Orthobunyavirus of the family Bunyaviridae. Phylogenetic analysis of L, M, S (N and NSs) revealed its close association with Chinese strain of Oya virus in Simbu serogroup with the distance of 6.5>4.2>3.2% for nucleotides and 2.4>0.8>0.0% for the amino acid of L>M>S segments respectively. Based on the PCR results; an isolate from pig sample was also confirmed as Oya virus. This study was strengthened by findings of IgG antibody positivity against Oya virus in retrospective serum samples of suspected febrile illness cases from this area by an indigenously developed ELISA. Oya virus positivity was also recorded in human samples collected from Karnataka using nested RT-PCR. This is the first report of the presence of Oya virus in human samples. Further studies are needed to determine disease-causing potential in humans.
1986年在印度卡纳塔克邦科拉尔区进行日本脑炎（JE）研究期间；从一头猪和一份人类血清样本中，在幼年瑞士白化小鼠体内获得了两种病毒分离株。为了对这些病毒分离株进行特性鉴定，将它们在非洲绿猴肾细胞系CCL-81细胞中进行传代培养。通过逆转录聚合酶链反应（RT-PCR）对这些病毒分离株进行黄病毒（日本脑炎、西尼罗河病毒、登革热病毒、基孔肯雅森林病病毒）和甲病毒（基孔肯雅病毒）筛查，结果均为阴性。进一步使用属特异性引物对它们进行布尼亚病毒筛查。使用下一代测序技术对一份人类样本中的病毒分离株进行测序；结果确定其为奥亚病毒，属于布尼亚病毒科正布尼亚病毒属辛布组。对L、M、S（N和NSs）基因片段进行系统发育分析表明，它与辛布血清群中的中国奥亚病毒株密切相关，L、M、S基因片段核苷酸的差异分别为6.5>4.2>3.2%，氨基酸差异分别为2.4>0.8>0.0%。基于聚合酶链反应（PCR）结果；来自猪样本的一个分离株也被确认为奥亚病毒。通过本土开发的酶联免疫吸附测定（ELISA），在该地区疑似发热疾病病例的回顾性血清样本中发现针对奥亚病毒的IgG抗体呈阳性，这进一步证实了该研究结果。使用巢式逆转录聚合酶链反应（nested RT-PCR）在从卡纳塔克邦采集的人类样本中也检测到了奥亚病毒阳性。这是人类样本中存在奥亚病毒的首次报告。需要进一步研究以确定其对人类致病的可能性。
