Lim Marisa C W, Seimon Anton, Nightingale Batya, Xu Charles C Y, Halloy Stephan R P, Solon Adam J, Dragone Nicholas B, Schmidt Steven K, Tait Alex, Elvin Sandra, Elmore Aurora C, Seimon Tracie A
Wildlife Conservation Society, Zoological Health Program, Bronx Zoo, Bronx, NY 10460, USA.
Appalachian State University, Department of Geography and Planning, Boone, NC 28608, USA.
iScience. 2022 Aug 15;25(9):104848. doi: 10.1016/j.isci.2022.104848. eCollection 2022 Sep 16.
Species composition in high-alpine ecosystems is a useful indicator for monitoring climatic and environmental changes at the upper limits of habitable environments. We used environmental DNA (eDNA) analysis to document the breadth of high-alpine biodiversity present on Earth's highest mountain, Mt. Everest (8,849 m a.s.l.) in Nepal's Khumbu region. In April-May 2019, we collected eDNA from ten ponds and streams between 4,500 m and 5,500 m. Using multiple sequencing and bioinformatic approaches, we identified taxa from 36 phyla and 187 potential orders across the Tree of Life in Mt. Everest's high-alpine and aeolian ecosystem. These organisms, all recorded above 4,500 m-an elevational belt comprising <3% of Earth's land surface-represents ∼16% of global taxonomic order estimates. Our eDNA inventory will aid future high-Himalayan biomonitoring and retrospective molecular studies to assess changes over time as climate-driven warming, glacial melt, and anthropogenic influences reshape this rapidly transforming world-renowned ecosystem.
高海拔生态系统中的物种组成是监测宜居环境上限处气候和环境变化的有用指标。我们利用环境DNA（eDNA）分析来记录地球上最高山峰——尼泊尔昆布地区的珠穆朗玛峰（海拔8849米）上高海拔生物多样性的广度。2019年4月至5月，我们从海拔4500米至5500米之间的10个池塘和溪流中采集了eDNA。通过多种测序和生物信息学方法，我们在珠穆朗玛峰的高海拔和风成生态系统中确定了来自36个门和187个潜在目、跨越生命之树的分类单元。这些生物均记录于海拔4500米以上——这一海拔带占地球陆地表面不到3%——约占全球分类目估计数的16%。我们的eDNA清单将有助于未来喜马拉雅地区的生物监测和回顾性分子研究，以评估随着气候驱动的变暖、冰川融化和人为影响重塑这个迅速变化的世界著名生态系统，生物多样性随时间的变化。
Zotero 插件
