Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA.
Methods Mol Biol. 2023;2576:21-40. doi: 10.1007/978-1-0716-2728-0_3.
Different mass spectrometric techniques have been used over the past decade to quantify endocannabinoids (eCBs) and related lipids. Even with the level of molecular fingerprinting accuracy of an instrument like the most advanced triple quadrupole mass spectrometer, if one is not getting the most optimized sample to the detector in a way that this improved technology can be of use, then advancements can be stymied. Here, our focus is on review and discussion of sample preparation methodologies used to isolate the eCB anandamide and its close congeners N-acyl ethanolamines and structural congeners (i.e., lipo amino acids, lipoamines, N-acyl amides) in biological fluids. Most of our focus will be on the analysis of these lipids in plasma/serum, but we will also discuss how the same techniques can be used for the analysis of saliva and breast milk.
在过去的十年中，人们已经使用了不同的质谱技术来定量内源性大麻素（eCBs）和相关脂质。即使仪器（如最先进的三重四极杆质谱仪）具有分子指纹识别的准确性，如果不能以最优化的方式将样品输送到检测器，那么这种改进的技术也无法发挥作用，进展可能会受到阻碍。在这里，我们的重点是回顾和讨论用于分离生物体液中的内源性大麻素大麻酰胺及其密切同系物 N-酰基乙醇胺和结构同系物（即脂氨基 酸、脂胺、N-酰基酰胺）的样品制备方法。我们的重点主要将放在分析这些脂质在血浆/血清中的含量上，但我们也将讨论相同的技术如何用于分析唾液和母乳。
