Université de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Institute of Biophysics, University of Natural Resources and Life Sciences Vienna, Vienna, Austria.
Methods Mol Biol. 2023;2566:261-268. doi: 10.1007/978-1-0716-2675-7_21.
In some specific vascular plant tissues, lignin can impregnate the entire cell wall to make it more rigid and hydrophobic. Different techniques have been developed in the past years to make possible the quantification of this polyphenolic polymer at the organ or tissue level, but difficulties of access to the cellular level remain. Here we describe an approach based on ratiometric emission measurements using safranin-O and the development of a macro adapted for the FIJI software, which makes it possible to quantify lignin in three different layers of the cell wall on images captured on a fluorescent confocal microscope.
在一些特定的维管植物组织中，木质素可以渗透整个细胞壁，使其更加坚硬和疏水。过去几年已经开发出不同的技术来实现对这种多酚聚合物在器官或组织水平上的定量，但进入细胞水平仍然存在困难。在这里，我们描述了一种基于使用磺基罗丹明 O 的比率发射测量的方法，并开发了一种适用于 FIJI 软件的宏观方法，这使得可以在荧光共聚焦显微镜上捕获的图像上对细胞壁的三个不同层中的木质素进行定量。
