Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Ramapuram, Chennai, 600087, India.
School of Biosciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Environ Res. 2022 Dec;215(Pt 3):114408. doi: 10.1016/j.envres.2022.114408. Epub 2022 Sep 23.
The current study demonstrated a green, friendly, low-cost biosynthesis of silver nanoparticles (AgNPs) from Kigelia africana leaves (Lam.) Benth. extract (KAE) as both a major capping and reducing agent. The produced AgNPs were characterized using a variety of analytical methods, like the X-ray powder diffraction (XRD), HRTEM, Fourier transforms infrared (FTIR), and UV-Vis spectrophotometer. The formation of AgNPs with maximum absorbance at max = 435 nm was endorsed by surface plasmon resonance. FTIR analysis revealed that biological macromolecules of KAE were involved in the stabilization and synthesis of AgNPs. At the same time, HRTEM images revealed that the average particle size of the spherical AgNPs ranged from about 25 nm to 35 nm. Further, cytotoxicity assessment of AgNPs was done using the RINm5F insulinoma cell line with an MTT assay. Followed by, the RINm5F insulinoma cells treated with AgNPs and KAE, the expression of the Peroxisome proliferator-activated receptor gamma (PPARγ) gene was accessed. The results showed gene expression was upregulated in the RINm5F insulinoma cell line thus confirming AgNPs and KAE anti-diabetic efficacy. Furthermore, the findings show that nanotechnology has enhanced the effectiveness of current methodologies in gene expression and regulation which has contributed to the emergence of different forms of advanced regulatory systems.
本研究展示了一种绿色、友好、低成本的银纳米粒子（AgNPs）的生物合成方法，使用非洲吊灯树（Kigelia africana Lam.）叶片提取物（KAE）作为主要的稳定剂和还原剂。通过多种分析方法，如 X 射线粉末衍射（XRD）、高分辨率透射电子显微镜（HRTEM）、傅里叶变换红外光谱（FTIR）和紫外-可见分光光度计对所制备的 AgNPs 进行了表征。表面等离子体共振（SPR）证实了具有最大吸收峰 max=435nm 的 AgNPs 的形成。FTIR 分析表明，KAE 的生物大分子参与了 AgNPs 的稳定和合成。同时，HRTEM 图像显示，球形 AgNPs 的平均粒径范围约为 25nm 至 35nm。此外，通过 MTT 测定法，使用 RINm5F 胰岛细胞瘤系评估了 AgNPs 的细胞毒性。接着，用 AgNPs 和 KAE 处理 RINm5F 胰岛细胞瘤系，检测过氧化物酶体增殖物激活受体γ（PPARγ）基因的表达。结果表明，RINm5F 胰岛细胞瘤系中基因表达上调，从而证实了 AgNPs 和 KAE 的抗糖尿病功效。此外，研究结果表明，纳米技术增强了当前基因表达和调控方法的有效性，这有助于出现不同形式的先进调控系统。
