温度影响污泥厌氧消化过程中1类整合子的变化。
Temperature affects variations of class 1 integron during sludge anaerobic digestion.
作者信息
Zhang Yan, Su Yu-Ao, Qiu Xuyang, Mao Qiuyan, Liu Hongbo, Liu He, Wen Donghui, Su Zhiguo
机构信息
School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Key Laboratory of Anaerobic Biotechnology, Wuxi 214122, China; Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou 215011, China.
School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.
出版信息
Bioresour Technol. 2022 Nov;364:128005. doi: 10.1016/j.biortech.2022.128005. Epub 2022 Sep 22.
Revealing class 1 integron characteristics under different operating conditions is of great importance to control antibiotic resistance genes (ARGs) during sludge anaerobic digestion (AD). This study investigated the variations of class 1 integrons and the ARGs carried by class 1 integrons in anaerobic sludge digesters under 25 °C, 35 °C, and 55 °C. The results showed lower intI1 abundance and fewer class I integrons with long gene cassette arrays at 55 °C than at 25 °C and 35 °C. Multi-resistance gene cassette arrays were observed in the digesters at 25 °C and 35 °C. Abundant ARGs were detected on class 1 integrons in all digesters with aminoglycosides as the dominant class. The abundance of ARGs on class 1 integrons in digesters at 55 °C was lower than that at 25 °C and 35 °C. Thermophilic AD is better than mesophilic ones in the control of ARGs carried by class 1 integrons.
揭示不同运行条件下1类整合子的特征对于在污泥厌氧消化（AD）过程中控制抗生素抗性基因（ARG）至关重要。本研究调查了25℃、35℃和55℃下厌氧污泥消化池中1类整合子及其携带的ARG的变化。结果表明，与25℃和35℃相比，55℃时intI1丰度较低，具有长基因盒阵列的I类整合子较少。在25℃和35℃的消化池中观察到多抗性基因盒阵列。在所有消化池中，1类整合子上检测到丰富的ARG，其中氨基糖苷类为主要类别。55℃消化池中1类整合子上ARG的丰度低于25℃和35℃。在控制1类整合子携带的ARG方面，高温厌氧消化优于中温厌氧消化。
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