Zhou Qian, Xu Wenqi, Xia Deju, Zhu Xiaoyu, Han Yan, Chen Kai, Yin Yueping
Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, People's Republic of China.
National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People's Republic of China.
Infect Drug Resist. 2022 Sep 18;15:5475-5481. doi: 10.2147/IDR.S381361. eCollection 2022.
The accurate detection of antibiotic susceptibility of () is of great importance for the treatment of patients with gonorrhea as well as to hinder the progress of drug resistance. To promote the application of gonococcal antibiotic susceptibility monitoring in primary hospitals and remote medical institutions, this study evaluated the effect of alternative growth supplements on the antibiotic susceptibility testing of isolates.
We divided the antimicrobial-containing media into three groups by adding different growth supplements (sterile defibrinated sheep blood, bovine hemoglobin, and Vitox). We tested the antimicrobial susceptibility of 80 isolates in different groups against eight antibiotics. Nonparametric signed-rank tests were utilized to compare the minimum inhibitory concentration (MIC) results of each group. Taking the MIC results of Vitox group as expected, the essential agreement (EA) and category agreement (CA) of the other two groups were calculated.
For the group using sheep blood as growth supplements, the EA values and CA values of each antibiotic were above 90.00% and minor error rates were less than 7.00%. No very major error and major error were observed. For the group using hemoglobin as growth supplements, the EA values of the susceptibility results of zoliflodacin, penicillin, and ceftriaxone were lower than 90.00%. The overall MIC results of using hemoglobin as a growth supplement were higher than those of sheep blood and Vitox in the susceptibility testing of these three antibiotics.
Compared with the expected results, sheep blood may be considered for the use as an alternative material for antibiotics susceptibility surveillance, while hemoglobin may not be suitable for supplement to antimicrobial-containing medium.
准确检测淋病奈瑟菌的抗生素敏感性对于淋病患者的治疗以及阻碍耐药性的发展至关重要。为促进基层医院和偏远医疗机构中淋球菌抗生素敏感性监测的应用，本研究评估了替代生长补充剂对淋病奈瑟菌分离株抗生素敏感性试验的影响。
我们通过添加不同的生长补充剂（无菌去纤维羊血、牛血红蛋白和Vitox）将含抗菌药物的培养基分为三组。我们测试了不同组中的80株淋病奈瑟菌分离株对八种抗生素的抗菌敏感性。采用非参数符号秩检验比较每组的最低抑菌浓度（MIC）结果。以Vitox组的MIC结果为预期，计算其他两组的基本一致率（EA）和类别一致率（CA）。
对于使用羊血作为生长补充剂的组，每种抗生素的EA值和CA值均高于90.00%，小错误率低于7.00%。未观察到极重大错误和重大错误。对于使用血红蛋白作为生长补充剂的组，左氟达西、青霉素和头孢曲松敏感性结果的EA值低于90.00%。在这三种抗生素的敏感性试验中，使用血红蛋白作为生长补充剂的总体MIC结果高于羊血和Vitox组。
与预期结果相比，羊血可被考虑用作淋病奈瑟菌抗生素敏感性监测的替代材料，而血红蛋白可能不适合补充到含抗菌药物的培养基中。
