Xu Wenqi, Zhou Qian, Liu Jingwei, Zhang Yan, Zhu Xiaoyu, Zhu Bangyong, Yin Yueping
Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China.
STD Reference Laboratory, National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing 210042, China.
Antibiotics (Basel). 2022 Aug 10;11(8):1083. doi: 10.3390/antibiotics11081083.
The susceptibility to gentamicin of isolates collected in three Chinese provinces and the correlation among the MICs of gentamicin, azithromycin, and ceftriaxone were investigated in this study. The effects of combinations from those three antibiotics were also in the scope of this study to determine the efficacy of gentamicin as a combination therapeutic drug. The agar dilution method was used to measure the minimum inhibitory concentrations (MICs) of ceftriaxone, azithromycin and gentamicin on isolates. The synergy between these three antimicrobials were determined using the agar dilution checkerboard method. Subgroup studies were conducted to explore differences between azithromycin- and ceftriaxone-sensitive and resistant isolates. A total of 139 (36.60%) and 233 (61.30%) isolates demonstrated full susceptibility and intermediate susceptibility to gentamicin, respectively. The correlation analysis showed that the MICs of ceftriaxone and azithromycin weakly correlated with the value of gentamicin. The overall results of the three antibiotic combinations revealed indifferent effects. Combination therapy established a significant reduction on the MIC value. Most of the isolates tested in this study demonstrated a certain degree of susceptibility to gentamicin. Overall, antimicrobial combinations of gentamicin with ceftriaxone or azithromycin demonstrate indifferent effects.
本研究调查了在中国三个省份收集的分离株对庆大霉素的敏感性，以及庆大霉素、阿奇霉素和头孢曲松的最低抑菌浓度（MIC）之间的相关性。这三种抗生素联合使用的效果也在本研究范围内，以确定庆大霉素作为联合治疗药物的疗效。采用琼脂稀释法测定头孢曲松、阿奇霉素和庆大霉素对分离株的最低抑菌浓度。使用琼脂稀释棋盘法确定这三种抗菌药物之间的协同作用。进行亚组研究以探索阿奇霉素和头孢曲松敏感及耐药分离株之间的差异。分别有139株（36.60%）和233株（61.30%）分离株对庆大霉素表现出完全敏感和中度敏感。相关性分析表明，头孢曲松和阿奇霉素的MIC与庆大霉素的值弱相关。三种抗生素联合使用的总体结果显示效果不佳。联合治疗使MIC值显著降低。本研究中测试的大多数分离株对庆大霉素表现出一定程度的敏感性。总体而言，庆大霉素与头孢曲松或阿奇霉素的抗菌联合效果不佳。
