Wang Yiming, Luo Wenqi, Lin Feng, Liu Wanguo, Gu Rui
Department of Orthopaedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
Front Bioeng Biotechnol. 2022 Sep 8;10:989602. doi: 10.3389/fbioe.2022.989602. eCollection 2022.
Spinal cord injury (SCI) is a severely crippling injury. Scavenging reactive oxygen species (ROS) and suppressing inflammation to ameliorate secondary injury using biomaterials has turned into a promising strategy for SCI recuperation. Herein, epigallocatechin-3-gallate selenium nanoparticles (EGCG-Se NP) that scavenge ROS and attenuate inflammation were used for neuroprotection in SCI. EGCG-Se NP were arranged using a simple redox framework. The size, morphology, and chemical structure of the EGCG-Se NP were characterized. The protective effect of EGCG-Se NP for neuroprotection was examined in cell culture and in an SCI rat model. EGCG-Se NP could promptly scavenge excess ROS and safeguard PC12 cells against HO-induced oxidative harm . After intravenous delivery in SCI rats, EGCG-Se NP significantly improved locomotor capacity and diminished the injury region by safeguarding neurons and myelin sheaths. Component studies showed that the main restorative impact of EGCG-Se NP was due to their ROS-scavenging and anti-inflammatory properties. This study showed the superior neuroprotective effect of EGCG-Se NP through ROS sequestration and anti-inflammatory capabilities. EGCG-Se NP could be a promising and effective treatment for SCI.
脊髓损伤（SCI）是一种严重致残的损伤。利用生物材料清除活性氧（ROS）并抑制炎症以改善继发性损伤已成为脊髓损伤恢复的一种有前景的策略。在此，用于清除ROS并减轻炎症的表没食子儿茶素-3-没食子酸酯硒纳米颗粒（EGCG-Se NP）被用于脊髓损伤的神经保护。EGCG-Se NP通过简单的氧化还原体系制备而成。对EGCG-Se NP的尺寸、形态和化学结构进行了表征。在细胞培养和脊髓损伤大鼠模型中检测了EGCG-Se NP对神经保护的作用。EGCG-Se NP能迅速清除过量的ROS，并保护PC12细胞免受过氧化氢诱导的氧化损伤。在脊髓损伤大鼠静脉注射后，EGCG-Se NP通过保护神经元和髓鞘显著提高了运动能力并缩小了损伤区域。成分研究表明，EGCG-Se NP的主要修复作用归因于其清除ROS和抗炎特性。本研究通过ROS清除和抗炎能力表明了EGCG-Se NP具有卓越的神经保护作用。EGCG-Se NP可能是一种有前景且有效的脊髓损伤治疗方法。
