Yin Hang, Wang Zhaoyu, Li Han, Zhang Yu, Yang Mei, Cui Guowen, Zhang Pan
Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Front Plant Sci. 2022 Sep 8;13:992024. doi: 10.3389/fpls.2022.992024. eCollection 2022.
In recent years, drought stress caused by global warming has become a major constraint on agriculture. The thiamine thiazole synthase (THI1) is responsible for controlling thiamine production in plants displaying a response to various abiotic stresses. Nonetheless, most of the THI1 activities in plants remain largely unknown. In this study, we extracted from alfalfa and demonstrated its beneficial impact on improving the resistance of plants to stress conditions. The highest levels of expression were identified in alfalfa leaves, triggered by exposure to cold, drought, salt, or alkaline conditions. The upregulation of in drought-stressed transgenic plants resulted in enhanced accumulation of vitamin B1 (VB1), chlorophyll (Chl ), chlorophyll (Chl ), soluble protein, higher soil and plant analyzer development (SPAD) value, and the activity of peroxidase (POD), maintained Fv/Fm, and decreased lipid peroxidation. Moreover, overexpression of upregulated the transcription of , and and downregulated the transcription of and in transgenic alfalfa under drought stress. These results suggested that enhances drought tolerance by strengthening photosynthesis, regulating the antioxidant defense system, maintaining osmotic homeostasis, and mediating plant signal transduction.
近年来，全球变暖导致的干旱胁迫已成为农业的主要制约因素。硫胺噻唑合酶（THI1）负责控制植物中硫胺素的产生，植物对各种非生物胁迫有响应。尽管如此，植物中大多数THI1的活性仍很大程度上未知。在本研究中，我们从苜蓿中提取了（此处原文缺失提取的物质名称），并证明了其对提高植物抗逆性的有益影响。苜蓿叶片中（此处原文缺失物质名称）的表达水平最高，在暴露于寒冷、干旱、盐或碱性条件下时被触发。干旱胁迫转基因植物中（此处原文缺失物质名称）的上调导致维生素B1（VB1）、叶绿素a（Chl a）、叶绿素b（Chl b）、可溶性蛋白的积累增加，土壤和植物分析仪发展（SPAD）值升高，过氧化物酶（POD）活性增强，维持Fv/Fm，并降低脂质过氧化。此外，在干旱胁迫下，（此处原文缺失物质名称）的过表达上调了转基因苜蓿中（此处原文缺失相关基因名称）、（此处原文缺失相关基因名称）的转录，并下调了（此处原文缺失相关基因名称）和（此处原文缺失相关基因名称）的转录。这些结果表明，（此处原文缺失物质名称）通过增强光合作用、调节抗氧化防御系统、维持渗透稳态和介导植物信号转导来提高耐旱性。
