高脂肪高果糖饮食降低 Sprague-Dawley 大鼠模型盲肠内容物重量和短链脂肪酸水平。

A high-fat and high-fructose diet lowers the cecal digesta's weight and short-chain fatty acid level of a Sprague-Dawley rat model.

机构信息

Department of Nutrition, Malang State Health Polytechnic Ministry of Health, Malang Indonesia.

School of Nutrition, Faculty of Medicine, Brawijaya University, Malang, Indonesia.

出版信息

Turk J Med Sci. 2022 Feb;52(1):268-275. doi: 10.3906/sag-1911-14. Epub 2022 Feb 22.

DOI:10.3906/sag-1911-14

PMID:36161602

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10734861/

Abstract

BACKGROUND

This study aimed to analyze the effect of a high-fat and high-fructose diet (HFFD) on the digesta weight and shortchain fatty acid (SCFA) levels of cecal digesta in rats.

METHODS

This study was an experimental study with a posttest-only control group design with male Sprague-Dawley strain rats as the samples. A total of 36 rats were divided into two groups with normal diet (N) and modified HFFD. The data of energy intake, nutrients and fiber, body weight, Lee index, abdominal circumference, digesta weight, and SCFA levels of cecal digesta were collected.

RESULTS

The results showed an 11.94% increase in body weights of rats with HFFD. The total energy intake of the HFFD group was significantly higher than that of N (p = 0.000). The fiber intake and cecal digesta weight in group N were higher than that in the HFFD group (p = 0.00 and p = 0.02, respectively). The concentrations of acetate, butyrate, propionate, and total SCFA in the N group were significantly higher than in the HFFD (p = 0.041,,p = 0.004, p = 0.040, p = 0.013, respectively). A significant negative relationship was observed between the abdominal circumference and cecal digesta concentration (p = 0.029; r = -0.529) and between the Lee index and the SCFA concentration of cecal digesta (p = 0.036, r = -0.206).

摘要

背景

本研究旨在分析高脂肪高果糖饮食（HFFD）对大鼠盲肠内容物重量和短链脂肪酸（SCFA）水平的影响。

方法

本研究是一项实验性研究，采用后测仅有对照组设计，以雄性 Sprague-Dawley 品系大鼠为样本。共 36 只大鼠分为正常饮食（N）和改良 HFFD 两组。收集能量摄入、营养素和纤维、体重、Lee 指数、腹围、盲肠内容物重量和盲肠内容物 SCFA 水平的数据。

结果

结果显示，HFFD 组大鼠体重增加了 11.94%。HFFD 组的总能量摄入量明显高于 N 组（p=0.000）。N 组的纤维摄入量和盲肠内容物重量均高于 HFFD 组（p=0.00 和 p=0.02）。N 组的乙酸、丁酸、丙酸和总 SCFA 浓度均明显高于 HFFD 组（p=0.041、p=0.004、p=0.040、p=0.013）。腹围与盲肠内容物浓度呈显著负相关（p=0.029；r=-0.529），Lee 指数与盲肠内容物 SCFA 浓度呈显著负相关（p=0.036，r=-0.206）。