Effect of the Extract on Methamphetamine-Induced Cytotoxicity in SH-SY5Y Cells.

This study aimed to assess the effect of the extract in methamphetamine-induced SH-SY5Y cells death. The brown algae S. was extracted with 80% ethanol. The SH-SY5Y cells were treated with different concentrations of methamphetamine to measure IC.. The MTT test was used to assess the toxic effect of the extract in SH-SY5Y cells. SH-SY5Y cells' survival was measured while cells were treated with different concentrations of methamphetamine and S. extract simultaneously. A specific kit measured intracellular ROS levels. Western blot analysis evaluated the expression of cytochrome C and Bax/Bcl2 ratio. The results showed that 5 mM methamphetamine approximately killed 50% of the cells, so it is considered IC. The MTT test showed no toxicity effect for the S. extract. 80, 160, 320, and 640 g/ml of S. extract prevented the occurrence of methamphetamine toxic effects in SH-SY5Y cells after 24 hours. Moreover, the extract decreased ROS levels and cytochrome C release and reduced BaX/Bcl2 ratio in cells treated by methamphetamine. On the whole, it seems that the S. hydroalcoholic extract has the potential to increase cell survival through antioxidant and antiapoptotic activities.