Department of Traditional Chinese Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Zhejiang Chinese Medical University, Hangzhou, China.
Sleep Breath. 2023 Jun;27(3):1143-1153. doi: 10.1007/s11325-022-02714-y. Epub 2022 Sep 27.
Raw "Pinelliae Rhizoma" (RPR) is widely used in Chinese clinics to treat insomnia. This study investigated its underlying sedative and hypnotic mechanisms and main active components.
A locomotor activity test was used to evaluate the sedative effects of RPR at three dosages (0.2 g/mL, 0.4 g/mL, and 0.8 g/mL) in mice. Polysomnography was used to assess its ability to improve sleep. Ultra-performance liquid chromatography/time of flight/mass spectrometry (UPLC-TOF-MS) analysis was used to identify the potential active components of RPR.
Mice in the RPR groups were less active than mice in the vehicle group; this difference was greatest in the 0.8 g/mL RPR group. Compared with the vehicle, 0.8 g/mL RPR increased the duration of rapid eye movement (REM) sleep in the dark phase. In addition, the duration of wakefulness in the 0.8 g/mL RPR group decreased with increasing durations of nonrapid eye movement (NREM) and REM sleep. Compared with diazepam, 0.8 g/mL RPR increased REM sleep duration in both the light and dark phases and increased the number of transitions both from NREM sleep to REM sleep and from REM sleep to wakefulness. A total of 33 RPR constituents, including 15 alkaloids, were identified.
The results preliminarily indicated that RPR exerts sedative and hypnotic effects in mice, mainly leading to improvements in REM sleep. These effects are possibly due to the alkaloid constituents of RPR.
生半夏在临床上广泛用于治疗失眠。本研究旨在探讨其潜在的镇静催眠作用机制及主要活性成分。
通过对小鼠进行自发活动实验，评估半夏生品在三个剂量（0.2 g/mL、0.4 g/mL 和 0.8 g/mL）下的镇静作用；通过多导睡眠图评估其改善睡眠的能力；采用超高效液相色谱飞行时间质谱联用技术（UPLC-TOF-MS）分析半夏生品的潜在活性成分。
半夏生品组小鼠的自发活动较溶剂组减少，其中以 0.8 g/mL 半夏生品组最为显著；与溶剂组相比，0.8 g/mL 半夏生品组增加了暗期快速眼动（REM）睡眠时间；此外，0.8 g/mL 半夏生品组的觉醒时间随着非快速眼动（NREM）和 REM 睡眠时间的增加而减少。与地西泮相比，0.8 g/mL 半夏生品组增加了光暗期的 REM 睡眠时间，并增加了从 NREM 睡眠向 REM 睡眠以及从 REM 睡眠向觉醒的转换次数。共鉴定出 33 个半夏生品成分，包括 15 个生物碱。
本研究初步表明，半夏生品对小鼠具有镇静催眠作用，主要表现为 REM 睡眠的改善，其作用可能与半夏生品的生物碱成分有关。
