Department of Traditional Chinese Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
Biomed Res Int. 2019 Apr 28;2019:6198067. doi: 10.1155/2019/6198067. eCollection 2019.
(PRPCA) is useful for eliminating dampness and phlegm in clinical settings, targeting the main mechanisms of insomnia as defined in traditional Chinese medicine. However, little is known regarding the sedative and hypnotic effects of PRPCA. In the present study, we examined the sedative effects of PRPCA via a locomotor activity test and aimed to determine the most appropriate concentration of PRPCA for achieving these effects. The strongest sedative effects were observed at a PRPCA concentration of 0.45 g/ml. In addition, we investigated the hypnotic effects of PRPCA and its role in promoting sleep via sleep monitoring and vigilance state analysis. PRPCA increased rapid eye movement (REM) sleep and non-REM (NREM) sleep while decreasing wakefulness. In addition, PRPCA decreased the number of bouts of wakefulness (16-32 s and 32-64 s) and increased the number of bouts of NREM sleep (128-256 s). Furthermore, we identified a total of 32 component compounds via chromatography and mass spectrometry. Hence, the current work provides valuable information regarding the sedative and hypnotic effects of PRPCA and its regulatory mechanisms in promoting sleep.
（PRPCA）在临床上有助于去除湿气和痰浊，针对中医定义的失眠的主要机制。然而，关于 PRPCA 的镇静和催眠作用知之甚少。在本研究中，我们通过运动活性测试来检验 PRPCA 的镇静作用，并旨在确定实现这些作用的 PRPCA 的最适浓度。在 PRPCA 浓度为 0.45g/ml 时观察到最强的镇静作用。此外，我们通过睡眠监测和警觉状态分析研究了 PRPCA 的催眠作用及其促进睡眠的作用。PRPCA 增加了快速眼动（REM）睡眠和非快速眼动（NREM）睡眠，同时减少了觉醒。此外，PRPCA 减少了觉醒（16-32 秒和 32-64 秒）的次数，并增加了 NREM 睡眠（128-256 秒）的次数。此外，我们通过色谱和质谱鉴定出总共 32 种成分化合物。因此，本工作提供了关于 PRPCA 的镇静和催眠作用及其促进睡眠的调节机制的有价值的信息。
