Co-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China.
J Agric Food Chem. 2022 Oct 12;70(40):12772-12783. doi: 10.1021/acs.jafc.2c05770. Epub 2022 Sep 27.
Nervonic acid (NA) is a monounsaturated very long-chain fatty acid (VLCFA) and has been identified with critical biological functions in medical and health care for brain development and injury repair. Yet, the approaches to producing NA from the sources of plants or animals continue to pose challenges to meet increasing market demand, as they are generally associated with high costs, a lack of natural resources, a long life cycle, and low production efficiency. The recent technological advance in metabolic engineering allows us to precisely engineer oleaginous microbes to develop high-content NA-producing strains, which has the potential to provide a possible solution to produce NA on a commercial fermentation scale. In this Review, the biosynthetic pathway, natural sources, and metabolic engineering of NA are summarized. The strategies of metabolic engineering that could be adopted to modify oleaginous yeast to produce NA are discussed in detail, providing the prospecting views for the microbial cells producing NA.
神经酸（NA）是一种单不饱和超长链脂肪酸（VLCFA），已被确定在脑发育和损伤修复的医学和保健领域具有关键的生物学功能。然而，从植物或动物来源生产 NA 的方法仍然难以满足不断增长的市场需求，因为它们通常与高成本、缺乏自然资源、长生命周期和低生产效率相关。代谢工程的最新技术进步使我们能够精确地对产油微生物进行工程改造，以开发高含量 NA 生产菌株，这有可能为商业发酵规模生产 NA 提供一种可行的解决方案。在这篇综述中，总结了 NA 的生物合成途径、天然来源和代谢工程。详细讨论了可以采用的代谢工程策略来修饰产油酵母以生产 NA，为生产 NA 的微生物细胞提供了展望。
