植物源蛋白混合物的摄入引起的肌肉蛋白质合成反应与健康年轻男性摄入等量牛奶蛋白无差异。

The Muscle Protein Synthetic Response to the Ingestion of a Plant-Derived Protein Blend Does Not Differ from an Equivalent Amount of Milk Protein in Healthy Young Males.

机构信息

TiFN, Wageningen, The Netherlands.

NUTRIM School of Nutrition and Translational Research in Metabolism, Department of Human Biology, Maastricht University Medical Centre+, Maastricht, The Netherlands.

出版信息

J Nutr. 2023 Jan 14;152(12):2734-2743. doi: 10.1093/jn/nxac222.

BACKGROUND

Plant-derived proteins are considered to have lesser anabolic properties when compared with animal-derived proteins. The attenuated rise in muscle protein synthesis rates following ingestion of plant-derived compared with animal-derived protein has been, at least partly, attributed to deficiencies in specific amino acids such as leucine, lysine, and/or methionine. Combining different plant-derived proteins could provide plant-derived protein blends with a more balanced amino acid profile.

OBJECTIVES

This study aimed to compare postprandial muscle protein synthesis rates following the ingestion of 30 g milk protein with a 30 g blend combining wheat, corn, and pea protein in healthy young men.

METHODS

In a randomized, double-blind, parallel-group design, 24 young males (aged 24 ± 4 y) received a primed continuous l-[ring-13C6]-phenylalanine infusion after which they ingested 30 g milk protein (MILK) or a 30 g plant-derived protein blend combining 15 g wheat, 7.5 g corn, and 7.5 g pea protein (PLANT-BLEND). Blood and muscle biopsies were collected frequently for 5 h to assess postprandial plasma amino acid profiles (secondary outcome) and subsequent muscle protein synthesis rates (primary outcome). Data were analyzed by 2-factor repeated measures ANOVA and 2-samples t tests.

RESULTS

MILK increased plasma essential amino acid concentrations more than PLANT-BLEND over the 5 h postprandial period (incremental AUC = 151 ± 31 compared with 79 ± 12 mmol·300 min·L-1, respectively; P < 0.001). Ingestion of both MILK and PLANT-BLEND increased myofibrillar protein synthesis rates (P < 0.001), with no significant differences between treatments (0.053 ± 0.013%/h and 0.064 ± 0.016%/h, respectively; P = 0.08).

CONCLUSIONS

Ingestion of 30 g plant-derived protein blend combining wheat-, corn-, and pea-derived protein increases muscle protein synthesis rates in healthy young males. The muscle protein synthetic response to the ingestion of 30 g of this plant-derived protein blend does not differ from the ingestion of an equivalent amount of a high-quality animal-derived protein.Clinical trial registry number for Nederlands Trial Register: NTR6548 (https://trialsearch.who.int/Trial2.aspx?TrialID=NTR6548).

背景

与动物源性蛋白质相比，植物源性蛋白质被认为具有较低的同化特性。与动物源性蛋白相比，植物源性蛋白摄入后肌肉蛋白合成率的上升幅度减弱，至少部分归因于亮氨酸、赖氨酸和/或蛋氨酸等特定氨基酸的缺乏。将不同的植物源性蛋白质组合在一起，可以为植物源性蛋白质混合物提供更平衡的氨基酸谱。

目的

本研究旨在比较健康年轻男性摄入 30 克牛奶蛋白与摄入 30 克包含小麦、玉米和豌豆蛋白的植物源性蛋白质混合物后餐后肌肉蛋白合成率。

方法

在一项随机、双盲、平行组设计中，24 名年轻男性（年龄 24 ± 4 岁）接受了持续的 l-[环-13C6]-苯丙氨酸脉冲输注，之后他们摄入了 30 克牛奶蛋白（MILK）或 30 克包含 15 克小麦、7.5 克玉米和 7.5 克豌豆蛋白的植物源性蛋白质混合物（PLANT-BLEND）。在 5 小时内频繁采集血液和肌肉活检样本，以评估餐后血浆氨基酸谱（次要结局）和随后的肌肉蛋白合成率（主要结局）。数据采用双因素重复测量方差分析和两样本 t 检验进行分析。

结果

与摄入植物源性蛋白质混合物相比，摄入牛奶蛋白在餐后 5 小时内更能增加血浆必需氨基酸浓度（增量 AUC 分别为 151 ± 31 与 79 ± 12 mmol·300 min·L-1，P < 0.001）。摄入牛奶蛋白和植物源性蛋白质混合物均能增加肌原纤维蛋白合成率（P < 0.001），但两种处理之间无显著差异（分别为 0.053 ± 0.013%/h 和 0.064 ± 0.016%/h，P = 0.08）。