Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
Sci Total Environ. 2023 Jan 20;857(Pt 1):159055. doi: 10.1016/j.scitotenv.2022.159055. Epub 2022 Sep 26.
Benzotriazole UV stabilizer-329 (UV-329) is frequently detected in various environmental and biological matrices. However, the toxicity effect on freshwater benthos induced by UV-329 has rarely been described. In this study, genotoxicity, apoptosis, oxidative stress, histopathological alterations, siphoning behavior, and bioaccumulation in the gill and digestive gland of Corbicula fluminea exposed to UV-329 at 10, 100, and 1000 μg/L for 21 days were investigated. Toxicity screening using transcriptomics confirmed that UV-329 preferentially stimulated cellular process-related pathways including gap junctions, apoptosis, phagosomes and necroptosis. The transcript levels of a large number of apoptosis genes were significantly upregulated. This apoptosis mechanism was further confirmed by the fact that UV-329 exposure significantly increased the percentage of apoptotic cells, activated caspase-3, -8, and -9, and affected the antioxidant enzyme activities. Following exposure to 1000 μg/L UV-329, significant histological alterations were reflected in the corrosion of cilia, cellular swelling of epithelial cells in the gills, degeneration of digestive tubules, and necrosis of epithelial cells in the digestive glands. These results may aid in elucidating the toxicity mechanism of UV329 in bivalves and evaluating the hazards of UV-329 in benthic ecosystems.
苯并三唑紫外线稳定剂 329（UV-329）经常在各种环境和生物基质中被检测到。然而，UV-329 对淡水底栖生物的毒性影响很少被描述。在这项研究中，研究了 329 在 10、100 和 1000μg/L 暴露 21 天后对铜锈环棱螺的遗传毒性、细胞凋亡、氧化应激、组织病理学改变、虹吸行为和鳃、消化腺中的生物累积。使用转录组学进行的毒性筛选证实，UV-329 优先刺激与细胞过程相关的途径，包括缝隙连接、细胞凋亡、吞噬体和坏死。大量凋亡基因的转录水平显著上调。这种凋亡机制进一步得到证实，因为 UV-329 暴露显著增加了凋亡细胞的百分比，激活了 caspase-3、-8 和 -9，并影响了抗氧化酶的活性。在暴露于 1000μg/L UV-329 后，鳃的纤毛腐蚀、上皮细胞细胞肿胀、消化管退化和消化腺上皮细胞坏死等明显的组织学改变反映了出来。这些结果可能有助于阐明 UV329 在双壳类动物中的毒性机制，并评估 UV-329 在底栖生态系统中的危害。
