• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于液体活检的生物样品中癌症衍生外泌体的有效分离：经典策略与创新发展

Effective Separation of Cancer-Derived Exosomes in Biological Samples for Liquid Biopsy: Classic Strategies and Innovative Development.

作者信息

Xie Yujiao, Xu Xiawei, Lin Jie, Xu Yanping, Wang Jing, Ren Yong, Wu Aiguo

机构信息

Cixi Institute of Biomedical Engineering International Cooperation Base of Biomedical Materials Technology and Application Chinese Academy of Science (CAS) Key Laboratory of Magnetic Materials and Devices and Zhejiang Engineering Research Center for Biomedical Materials Ningbo Institute of Materials Technology and Engineering CAS Ningbo 315201 P. R. China.

Advanced Energy Science and Technology Guangdong Laboratory Huizhou 516000 P. R. China.

出版信息

Glob Chall. 2022 May 9;6(9):2100131. doi: 10.1002/gch2.202100131. eCollection 2022 Sep.

DOI:10.1002/gch2.202100131
PMID:36176940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9463520/
Abstract

Liquid biopsy has remarkably facilitated clinical diagnosis and surveillance of cancer via employing a non-invasive way to detect cancer-derived components, such as circulating tumor DNA and circulating tumor cells from biological fluid samples. The cancer-derived exosomes, which are nano-sized vesicles secreted by cancer cells have been investigated in liquid biopsy as their important roles in intracellular communication and disease development have been revealed. Given the challenges posed by the complicated humoral microenvironment, which contains a variety of different cells and macromolecular substances in addition to the exosomes, it has attracted a large amount of attention to effectively isolate exosomes from collected samples. In this review, the authors aim to analyze classic strategies for separation of cancer-derived exosomes, giving an extensive discussion of advantages and limitations of these methods. Furthermore, the innovative multi-strategy methods to realize efficient isolation of cancer-derived exosomes in practical applications are also presented. Additionally, the possible development trends of exosome separation in to the future is discussed in this review.

摘要

液体活检通过采用非侵入性方法来检测源自癌症的成分，如生物流体样本中的循环肿瘤DNA和循环肿瘤细胞，极大地促进了癌症的临床诊断和监测。癌症来源的外泌体是癌细胞分泌的纳米级囊泡，由于其在细胞内通讯和疾病发展中的重要作用已被揭示，因此在液体活检中受到了研究。鉴于复杂的体液微环境带来的挑战，除了外泌体之外，该微环境还包含各种不同的细胞和大分子物质，因此如何从收集的样本中有效分离外泌体引起了广泛关注。在这篇综述中，作者旨在分析分离癌症来源外泌体的经典策略，对这些方法的优缺点进行广泛讨论。此外，还介绍了在实际应用中实现高效分离癌症来源外泌体的创新多策略方法。此外，本综述还讨论了外泌体分离未来可能的发展趋势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/44d2cc6923dd/GCH2-6-2100131-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/d581b188a064/GCH2-6-2100131-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/99415ca0ca07/GCH2-6-2100131-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/3f6a30cf6637/GCH2-6-2100131-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/35a8a6ce5bb1/GCH2-6-2100131-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/f3ea08822baa/GCH2-6-2100131-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/d06ae080684a/GCH2-6-2100131-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/6dadcc96914e/GCH2-6-2100131-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/9553a7c87cee/GCH2-6-2100131-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/dbdf976260d3/GCH2-6-2100131-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/3bc8e89dc2f9/GCH2-6-2100131-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/b03c3b4b6b00/GCH2-6-2100131-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/44d2cc6923dd/GCH2-6-2100131-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/d581b188a064/GCH2-6-2100131-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/99415ca0ca07/GCH2-6-2100131-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/3f6a30cf6637/GCH2-6-2100131-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/35a8a6ce5bb1/GCH2-6-2100131-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/f3ea08822baa/GCH2-6-2100131-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/d06ae080684a/GCH2-6-2100131-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/6dadcc96914e/GCH2-6-2100131-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/9553a7c87cee/GCH2-6-2100131-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/dbdf976260d3/GCH2-6-2100131-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/3bc8e89dc2f9/GCH2-6-2100131-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/b03c3b4b6b00/GCH2-6-2100131-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a12d/9463520/44d2cc6923dd/GCH2-6-2100131-g012.jpg

相似文献

1
Effective Separation of Cancer-Derived Exosomes in Biological Samples for Liquid Biopsy: Classic Strategies and Innovative Development.用于液体活检的生物样品中癌症衍生外泌体的有效分离：经典策略与创新发展
Glob Chall. 2022 May 9;6(9):2100131. doi: 10.1002/gch2.202100131. eCollection 2022 Sep.
2
Exosome-based liquid biopsies in cancer: opportunities and challenges.基于外泌体的癌症液体活检：机遇与挑战。
Ann Oncol. 2021 Apr;32(4):466-477. doi: 10.1016/j.annonc.2021.01.074. Epub 2021 Feb 4.
3
Cancer Liquid Biopsy Using Integrated Microfluidic Exosome Analysis Platforms.癌症液体活检采用集成微流控外泌体分析平台。
Biotechnol J. 2020 May;15(5):e1900225. doi: 10.1002/biot.201900225. Epub 2020 Feb 20.
4
Review on Strategies and Technologies for Exosome Isolation and Purification.外泌体分离与纯化的策略与技术综述
Front Bioeng Biotechnol. 2022 Jan 5;9:811971. doi: 10.3389/fbioe.2021.811971. eCollection 2021.
5
Exosomes as a new frontier of cancer liquid biopsy.外泌体作为癌症液体活检的新前沿。
Mol Cancer. 2022 Feb 18;21(1):56. doi: 10.1186/s12943-022-01509-9.
6
A Snapshot of The Tumor Microenvironment in Colorectal Cancer: The Liquid Biopsy.结直肠癌肿瘤微环境概述：液体活检。
Int J Mol Sci. 2019 Nov 29;20(23):6016. doi: 10.3390/ijms20236016.
7
Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges.不同外泌体亚群的分离：分离和鉴定方法及其相关挑战。
Analyst. 2021 Jun 14;146(12):3731-3749. doi: 10.1039/d1an00024a.
8
Microfluidic-Based Exosome Analysis for Liquid Biopsy.基于微流控的外泌体分析用于液体活检。
Small Methods. 2021 Mar;5(3):e2001131. doi: 10.1002/smtd.202001131. Epub 2021 Jan 18.
9
Towards Microfluidic-Based Exosome Isolation and Detection for Tumor Therapy.用于肿瘤治疗的基于微流控的外泌体分离与检测
Nano Today. 2021 Apr;37. doi: 10.1016/j.nantod.2020.101066. Epub 2021 Jan 13.
10
Emerging role of exosomes as a liquid biopsy tool for diagnosis, prognosis & monitoring treatment response of communicable & non-communicable diseases.外泌体作为一种液体活检工具在传染性和非传染性疾病的诊断、预后和治疗反应监测中的新兴作用。
Indian J Med Res. 2024 Feb 1;159(2):163-180. doi: 10.4103/ijmr.ijmr_2344_22. Epub 2024 Apr 4.

引用本文的文献

1
Alternating Magnetic Field-Promoted Nanoparticle Mixing: The On-Chip Immunocapture of Serum Neuronal Exosomes for Parkinson's Disease Diagnostics.交变磁场促进纳米颗粒混合：用于帕金森病诊断的血清神经元外泌体的片上免疫捕获。
Anal Chem. 2023 May 23;95(20):7906-7913. doi: 10.1021/acs.analchem.3c00357. Epub 2023 May 11.

本文引用的文献

1
Coupling Nanostructured Microchips with Covalent Chemistry Enables Purification of Sarcoma-Derived Extracellular Vesicles for Downstream Functional Studies.将纳米结构微芯片与共价化学相结合可实现肉瘤衍生细胞外囊泡的纯化，用于下游功能研究。
Adv Funct Mater. 2020 Dec 1;30(49). doi: 10.1002/adfm.202003237. Epub 2020 Sep 13.
2
A Review on Deterministic Lateral Displacement for Particle Separation and Detection.用于粒子分离与检测的确定性侧向位移综述
Nanomicro Lett. 2019 Sep 17;11(1):77. doi: 10.1007/s40820-019-0308-7.
3
Simple and fast isolation of circulating exosomes with a chitosan modified shuttle flow microchip for breast cancer diagnosis.
利用壳聚糖修饰的穿梭流微芯片简单快速分离循环外泌体用于乳腺癌诊断。
Lab Chip. 2021 May 4;21(9):1759-1770. doi: 10.1039/d0lc01311k.
4
Liquid Biopsies: Applications for Cancer Diagnosis and Monitoring.液体活检：癌症诊断和监测的应用。
Genes (Basel). 2021 Feb 27;12(3):349. doi: 10.3390/genes12030349.
5
Exosome-based liquid biopsies in cancer: opportunities and challenges.基于外泌体的癌症液体活检：机遇与挑战。
Ann Oncol. 2021 Apr;32(4):466-477. doi: 10.1016/j.annonc.2021.01.074. Epub 2021 Feb 4.
6
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.《全球癌症统计数据 2020：全球 185 个国家和地区 36 种癌症的发病率和死亡率估计》。
CA Cancer J Clin. 2021 May;71(3):209-249. doi: 10.3322/caac.21660. Epub 2021 Feb 4.
7
Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation.开发用于高效癌症免疫化学和基于免疫亲和的外泌体分离的 CD63 Aptamer。
Molecules. 2020 Nov 27;25(23):5585. doi: 10.3390/molecules25235585.
8
Microfluidic Raman biochip detection of exosomes: a promising tool for prostate cancer diagnosis.微流控拉曼生物芯片检测外泌体：前列腺癌诊断的有前途的工具。
Lab Chip. 2020 Dec 15;20(24):4632-4637. doi: 10.1039/d0lc00677g.
9
Plasma Small Extracellular Vesicles Derived miR-21-5p and miR-92a-3p as Potential Biomarkers for Hepatocellular Carcinoma Screening.血浆来源的小细胞外囊泡中的miR-21-5p和miR-92a-3p作为肝细胞癌筛查的潜在生物标志物
Front Genet. 2020 Jul 23;11:712. doi: 10.3389/fgene.2020.00712. eCollection 2020.
10
Tangential flow microfluidics for the capture and release of nanoparticles and extracellular vesicles on conventional and ultrathin membranes.用于在常规膜和超薄膜上捕获和释放纳米颗粒及细胞外囊泡的切向流微流控技术
Adv Mater Technol. 2019 Nov;4(11). doi: 10.1002/admt.201900539. Epub 2019 Sep 20.