Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China.
Huizhou Third People's Hospital, Guangzhou Medical University, Huizhou 516002, China.
Food Chem. 2023 Mar 1;403:134391. doi: 10.1016/j.foodchem.2022.134391. Epub 2022 Sep 26.
In this study, we qualitatively and quantitatively characterized anthocyanins from 61 different Lonicera caerulea L. genotypes harvested from northeast China and measured their reactive oxygen species (ROS) scavenging activity. Among the anthocyanin extracts obtained, nine anthocyanins were identified, and the anthocyanin compositions could be divided into three classes and seven subtypes according to their numbers and distribution. Furthermore, their total content by fresh weight was ranged from 158.44 mg/100 g to 1751.44 mg/100 g across all genotypes; their hydroxyl radical scavenging activity and superoxide anion scavenging activity ranged from 13.3 %-99.9 % and 21.5 %-82.8 %, respectively. We also deployed Grey Relational Analysis to determine which anthocyanin was the key antioxidant component in the nine anthocyanins. The relation between the ROS scavenging activity and the 9 anthocyanin content was established. Our results suggest cyanidin-3,5-diglucoside has the greatest correlation to the ROS scavenging activity of the anthocyanin extractions.
在这项研究中，我们定性和定量地描述了从中国东北收获的 61 种不同的蓝靛果 Lonicera caerulea L. 基因型的花色苷，并测量了它们的活性氧（ROS）清除活性。在所获得的花色苷提取物中，鉴定出了九种花青素，根据其数量和分布，花色苷组成可分为三类和七类亚型。此外，所有基因型的花色苷总含量按鲜重计从 158.44 mg/100 g 到 1751.44 mg/100 g 不等；它们的羟基自由基清除活性和超氧阴离子清除活性分别为 13.3%-99.9%和 21.5%-82.8%。我们还采用灰色关联分析来确定九种花青素中哪种是关键的抗氧化成分。建立了 ROS 清除活性与 9 种花青素含量之间的关系。我们的结果表明，矢车菊素-3,5-二葡萄糖苷与花色苷提取物的 ROS 清除活性相关性最大。
