泰国九个省份啮齿动物和鼩鼱中分离出三种新物种的首次报告。

First report of three novel species isolated in rodents and shrews from nine provinces of Thailand.

作者信息

Pangjai Decha, Nimsuphan Burin, Petkanchanapong Wimol, Wootta Wattanapong, Boonyareth Maskiet, Rodkvamtook Wuttikon, Boonmar Sumalee

机构信息

Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.

Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

出版信息

Vet World. 2022 Jul;15(7):1624-1631. doi: 10.14202/vetworld.2022.1624-1631. Epub 2022 Jul 8.

DOI:10.14202/vetworld.2022.1624-1631

PMID:36185510

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9394139/

Abstract

BACKGROUND AND AIM

spp. are Gram-negative zoonotic bacteria that are transmitted to humans by several types of animal hosts, including rodents. Several studies have been conducted on the prevalence of infections in rodents. However, the risk of rodent-associated spp. infection in humans remains unclear. This study aimed to estimate the prevalence and genetic heterogeneity of spp. in rodents and shrews from nine provinces of Thailand using culture and molecular techniques.

MATERIALS AND METHODS

A total of 860 blood samples from rodents and shrews across nine provinces of Thailand were collected from January 2013 to June 2016. spp. were isolated from all samples using conventional culture techniques and polymerase chain reaction. Phylogenetic tree analysis was used to align the sequences obtained from this study.

RESULTS

The prevalence of spp. in rodents and shrews was 11.5% (99/860, 95% confidence interval: 9.38-13.64%). The following nine species of were detected: , , , , spp. nov., spp. nov., , spp. nov., and . The prevalence of positive animals differed significantly among provinces.

CONCLUSION

To the best of our knowledge, the three novel spp. isolated from rodents and shrews across Thailand were detected for the first time in this study. Further studies on the epidemiology of infection in rodents and its interaction with human health should be conducted in accordance with the Thai government's "One Health" approach to humans, animals, and the environment.

摘要

背景与目的

[细菌名称]属革兰氏阴性人畜共患病菌，可通过包括啮齿动物在内的多种动物宿主传播给人类。已针对啮齿动物中[细菌名称]感染的患病率开展了多项研究。然而，啮齿动物相关的[细菌名称]属感染对人类的风险仍不明确。本研究旨在运用培养和分子技术估算泰国九个省份的啮齿动物和鼩鼱中[细菌名称]属的患病率及基因异质性。

材料与方法

2013年1月至2016年6月期间，从泰国九个省份的啮齿动物和鼩鼱中总共采集了860份血液样本。使用传统培养技术和聚合酶链反应从所有样本中分离出[细菌名称]属。系统发育树分析用于比对本研究获得的[细菌名称]序列。

结果

啮齿动物和鼩鼱中[细菌名称]属的患病率为11.5%（99/860，95%置信区间：9.38 - 13.64%）。检测到以下九种[细菌名称]：[具体细菌种类1]、[具体细菌种类2]、[具体细菌种类3]、[具体细菌种类4]、[新细菌种类1]、[新细菌种类2]、[具体细菌种类5]、[新细菌种类3]和[具体细菌种类6]。各省阳性动物的患病率差异显著。