Winoto Imelda L, Goethert Heidi, Ibrahim Ima Nurisa, Yuniherlina Ikke, Stoops Craig, Susanti Ika, Kania Winny, Maguire Jason D, Bangs Michael J, Telford Sam R, Wongsrichanalai Chansuda
U.S. Naval Medical Research Unit No. 2, Jakarta, Indonesia.
Southeast Asian J Trop Med Public Health. 2005 Nov;36(6):1523-9.
In February 2004, we captured 221 rodents and shrews in the Greater Jakarta area as part of a study to determine the prevalence of rodent-associated vector-borne infections. Microscopic examination of blood smears revealed 6% (13/218) to be positive for Bartonella spp. The corresponding DNA samples, either from blood blots or frozen spleen pieces and from fleas collected on these animals, were tested for evidence of Bartonella infection by PCR, targeting the portions: 378bp and 930bp of the citrate synthase gene (g/tA). The sequences from our sample clusters with a Peruvian entity, B. phoceensis, B. rattimassiliensis and B. elizabethae, the latter species has been associated with endocarditis and neuroretinitis in humans. As previous analyses have shown, there appears to be little geographic or host consistency with phylogenetic placement. The public health significance of these findings remains to be determined.
2004年2月，作为一项确定啮齿动物相关媒介传播感染患病率研究的一部分，我们在大雅加达地区捕获了221只啮齿动物和鼩鼱。血液涂片的显微镜检查显示，6%（13/218）的样本对巴尔通体属呈阳性。对相应的DNA样本（来自血斑或冷冻脾脏碎片以及从这些动物身上采集的跳蚤）进行PCR检测，以寻找巴尔通体感染的证据，PCR靶向柠檬酸合酶基因（g/tA）的378bp和930bp部分。我们样本的序列与一个秘鲁菌株、滨海巴尔通体、鼠马赛尔巴尔通体和伊丽莎白巴尔通体聚类，后者已与人类的心内膜炎和神经视网膜炎有关。如先前分析所示，系统发育定位在地理或宿主方面似乎没有什么一致性。这些发现对公共卫生的意义仍有待确定。
