Liu Jie, Wu Yupo, Cui Xiaobo, Zhang Xiong, Xie Meili, Liu Lijiang, Liu Yueying, Huang Junyan, Cheng Xiaohui, Liu Shengyi
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of the PRC, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.
Front Plant Sci. 2022 Sep 14;13:962592. doi: 10.3389/fpls.2022.962592. eCollection 2022.
Ovate family proteins (OFPs) were firstly identified in tomato as proteins controlling the pear shape of the fruit. Subsequent studies have successively proved that OFPs are a class of negative regulators of plant development, and are involved in the regulation of complex traits in different plants. However, there has been no report about the functions of OFPs in rapeseed growth to date. Here, we identified the OFPs in rapeseed at the genomic level. As a result, a total of 67 members were obtained. We then analyzed the evolution from to , illustrated their phylogenetic and syntenic relationships, and compared the gene structure and conserved domains between different copies. We also analyzed their expression patterns in rapeseed, and found significant differences in the expression of different members and in different tissues. Additionally, we performed a GWAS for the number of seeds per silique (NSPS) in a rapeseed population consisting of 204 natural accessions, and identified a new gene significantly associated with NSPS, which was identified as a novel function of OFPs. Haplotype analysis revealed that the accessions with haplotype 3 had a higher NSPS than other accessions, suggesting that is associated with NSPS. Transcript profiling during the five stages of silique development demonstrated that negatively regulates NSPS. These findings provide evidence for functional diversity of OFP gene family and important implications for oilseed rape breeding.
卵形家族蛋白（OFPs）最初在番茄中被鉴定为控制果实梨形的蛋白质。随后的研究相继证明，OFPs是一类植物发育的负调控因子，并参与不同植物复杂性状的调控。然而，迄今为止，尚未有关于OFPs在油菜生长中功能的报道。在此，我们在基因组水平上鉴定了油菜中的OFPs。结果，共获得了67个成员。然后，我们分析了其进化过程，阐述了它们的系统发育和共线性关系，并比较了不同拷贝之间的基因结构和保守结构域。我们还分析了它们在油菜中的表达模式，发现不同成员在不同组织中的表达存在显著差异。此外，我们对由204份自然种质组成的油菜群体的每角果粒数（NSPS）进行了全基因组关联研究（GWAS），并鉴定出一个与NSPS显著相关的新基因，该基因被确定为OFPs的一个新功能。单倍型分析表明，单倍型3的种质比其他种质具有更高的NSPS，这表明该基因与NSPS相关。角果发育五个阶段的转录谱分析表明，该基因负调控NSPS。这些发现为OFPs基因家族的功能多样性提供了证据，并对油菜育种具有重要意义。
