Qiu Min, Zong Jia-Bin, He Quan-Wei, Liu Yu-Xiao, Wan Yan, Li Man, Zhou Yi-Fan, Wu Jie-Hong, Hu Bo
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Aging Dis. 2022 Oct 1;13(5):1436-1454. doi: 10.14336/AD.2022.0212.
Ischemic stroke is a detrimental neurological disease characterized by an irreversible infarct core surrounded by an ischemic penumbra, a salvageable region of brain tissue. Unique roles of distinct brain cell subpopulations within the neurovascular unit and peripheral immune cells during ischemic stroke remain elusive due to the heterogeneity of cells in the brain. Single-cell RNA sequencing (scRNA-seq) allows for an unbiased determination of cellular heterogeneity at high-resolution and identification of cell markers, thereby unveiling the principal brain clusters within the cell-type-specific gene expression patterns as well as cell-specific subclusters and their functions in different pathways underlying ischemic stroke. In this review, we have summarized the changes in differentiation trajectories of distinct cell types and highlighted the specific pathways and genes in brain cells that are impacted by stroke. This review is expected to inspire new research and provide directions for investigating the potential pathological mechanisms and novel treatment strategies for ischemic stroke at the level of a single cell.
缺血性中风是一种有害的神经系统疾病，其特征是存在一个不可逆的梗死核心，周围环绕着一个缺血半暗带，即一个可挽救的脑组织区域。由于大脑中细胞的异质性，神经血管单元内不同脑细胞亚群和外周免疫细胞在缺血性中风期间的独特作用仍不清楚。单细胞RNA测序（scRNA-seq）能够在高分辨率下无偏倚地确定细胞异质性并识别细胞标志物，从而揭示细胞类型特异性基因表达模式内的主要脑簇以及细胞特异性亚簇及其在缺血性中风潜在不同通路中的功能。在这篇综述中，我们总结了不同细胞类型分化轨迹的变化，并强调了受中风影响的脑细胞中的特定通路和基因。这篇综述有望激发新的研究，并为在单细胞水平上研究缺血性中风的潜在病理机制和新的治疗策略提供方向。
