Li Chunmei, Xiong Zhiwen, Fang Changran, Liu Kai
Guangzhou Key Laboratory for Research and Development of Crop Germplasm Resources, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Front Physiol. 2022 Sep 16;13:1018470. doi: 10.3389/fphys.2022.1018470. eCollection 2022.
The brown planthopper (BPH) (Stål) (Hemiptera: Delphacidae) is one of the most destructive rice pests in Asia. The application of insect-resistant rice cultivars is currently one of the principal means of controlling BPH. Understanding the physiological response mechanisms of BPH feeding on insect-resistant rice is the key for maintaining rice yield. Here, we measured the ecological fitness and analyzed the whole-body transcriptome and metabolome of BPH reared on susceptible cultivar Taichung Native 1 (TN1) and resistant cultivar Rathu Heenati (RH). Our results showed that RH significantly decreased the survival rate, female adult weight, honeydew secretion, the number of eggs laid per female and fat content of BPH. We identified 333 upregulated and 486 downregulated genes in BPH feeding on RH. These genes were mainly involved in energy metabolism, amino acid metabolism, hormone synthesis and vitamin metabolism pathways. We also detected 145 differentially accumulated metabolites in BPH reared on RH plants compared to BPH reared on TN1 plants, including multiple carbohydrates, amino acids, lipids, and some nucleosides. Combined analyses of transcriptome and metabolome showed that five pathways, including starch, sucrose, and galactose metabolism, were altered. The network for these pathways was subsequently visualized. Our results provide insights into the mechanisms of metabolite accumulation in BPH feeding on the RH rice variety. The results could help us better understand how insect-resistant rice cultivars combat BPH infestation, which is important for the comprehensive management of BPH.
褐飞虱（BPH）（斯塔尔）（半翅目：飞虱科）是亚洲最具破坏性的水稻害虫之一。应用抗虫水稻品种是目前控制褐飞虱的主要手段之一。了解褐飞虱取食抗虫水稻的生理反应机制是维持水稻产量的关键。在此，我们测量了在感虫品种台中本地1号（TN1）和抗病品种拉图希纳蒂（RH）上饲养的褐飞虱的生态适合度，并分析了其全身体转录组和代谢组。我们的结果表明，RH显著降低了褐飞虱的存活率、雌成虫体重、蜜露分泌量、每雌产卵数和脂肪含量。我们在取食RH的褐飞虱中鉴定出333个上调基因和486个下调基因。这些基因主要参与能量代谢、氨基酸代谢、激素合成和维生素代谢途径。与在TN1植株上饲养的褐飞虱相比，我们还在RH植株上饲养的褐飞虱中检测到145种差异积累的代谢物，包括多种碳水化合物、氨基酸、脂质和一些核苷。转录组和代谢组的联合分析表明，包括淀粉、蔗糖和半乳糖代谢在内的五条途径发生了改变。随后对这些途径的网络进行了可视化。我们的结果为取食RH水稻品种的褐飞虱中代谢物积累的机制提供了见解。这些结果有助于我们更好地理解抗虫水稻品种如何对抗褐飞虱的侵害，这对褐飞虱的综合管理很重要。
