Chemistry Department, Faculty of Science, Menoufia University, Menoufia, Egypt.
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Environ Sci Pollut Res Int. 2023 Feb;30(7):17374-17383. doi: 10.1007/s11356-022-23312-1. Epub 2022 Oct 4.
This study was designed to evaluate the protective potentials of chitosan nanoparticles (ChNPs) against silver nanoparticle (AgNP)-induced reproductive toxicity in male Wister albino rats. AgNPs, ChNPs, and AgNPs particles coated with ChNPs were characterized by using transmission electron microscope. Control rats were injected interperitoneally with 0.5% aqueous carboxymethyl cellulose. Second group was given ChNPs at a dose 300 mg/kg bwt. Third group was given AgNPs at a dose 50 mg/kg bwt. Fourth group was given AgNPs with chitosan nanoparticles simultaneously. Fifth group was given silver nanoparticles coated with chitosan nanoparticles at a dose 300 mg/kg bwt. TEM showed the formation of AgNPs with average size of 42.7 nm, ChNPs with average size of 33.3 nm, and AgNPs coated with ChNPs with average size of 48.1 nm. AgNPs significantly reduced serum levels of FSH, LH, testosterone and prolactin, sperm count, morphology index, vitality, total motility and progressive motility, the activities of catalase and superoxide dismutase, and the concentration of reduced glutathione in testicular tissues. However, it significantly increased malondialdehyde concentration in testicular tissues, sperm abnormalities, testicular tissue damages, non-progressive motility, and immotile sperms. On the contrast, ChNPs ameliorated AgNP-induced alteration in serum levels of sex hormones, spermogram, and testicular tissue's structure and functions. These results indicated that ChNPs had protective potential against AgNP-induced reproductive toxicity and ChNPs coating AgNPs had more potent protective effect than ChNPs administrated together with AgNPs.
本研究旨在评估壳聚糖纳米粒子（ChNPs）对雄性 Wister 白化大鼠中银纳米颗粒（AgNP）诱导的生殖毒性的保护潜力。使用透射电子显微镜对 AgNPs、ChNPs 和用 ChNPs 涂覆的 AgNPs 颗粒进行了表征。对照组大鼠腹膜内注射 0.5%羧甲基纤维素水溶液。第二组给予 300mg/kg bwt 的 ChNPs。第三组给予 50mg/kg bwt 的 AgNPs。第四组同时给予 AgNPs 和壳聚糖纳米粒子。第五组给予 300mg/kg bwt 的用壳聚糖纳米粒子涂覆的银纳米粒子。TEM 显示 AgNPs 的平均粒径为 42.7nm，ChNPs 的平均粒径为 33.3nm，AgNPs 用壳聚糖纳米粒子涂覆的平均粒径为 48.1nm。AgNPs 显著降低了血清中 FSH、LH、睾酮和催乳素、精子计数、形态指数、活力、总活力和渐进性活力、过氧化氢酶和超氧化物歧化酶的活性以及睾丸组织中还原型谷胱甘肽的浓度。然而，它显著增加了睾丸组织中丙二醛的浓度、精子异常、睾丸组织损伤、非渐进性活力和不动精子。相比之下，ChNPs 改善了 AgNP 诱导的血清性激素、精子图和睾丸组织结构和功能的改变。这些结果表明，ChNPs 具有对抗 AgNP 诱导的生殖毒性的保护潜力，并且用 ChNPs 涂覆 AgNPs 比同时给予 AgNPs 和 ChNPs 具有更强的保护作用。
