Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research, Guwahati, Changsari 781101, India.
Centre for GMP extraction Facility, National Institute of Pharmaceutical Education and Research, Guwahati, Changsari 781101, India.
Food Res Int. 2022 Nov;161:111874. doi: 10.1016/j.foodres.2022.111874. Epub 2022 Aug 28.
Phlogacanthus thyrsiformis (P. thyrsiformis) is a non-conventional edible plant that has been used as a vegetable and as a traditional medicine to treat various diseases. This non-conventional edible plant is widespread in India, Yunnan-Chinese provinces, and Vietnam. Despite this potential claim, the phytochemical investigation of plants remains incomplete. In the present study, the chemical profile analysis of P. thyrsiformis leaves extracts was performed using LC-ESI-QTOF-MS/MS and GC/QTOF-MS and assessed for its antioxidants and enzyme inhibitory potential. A cursory examination of the phytometabolites of different solvents extracts revealed chloroform extract has different metabolites, including phenols, terpenes, glycosides and alkaloids. 113 and 138 metabolites, including primary and secondary metabolites, were identified using LC-ESI-QTOF-MS/MS and GC/QTOF-MS, respectively. Total phenolic content was observed highest in chloroform extract (60.59 ± 1.25 mg GAE/g). Maximum antioxidant activity was found to be in chloroform extract, which was identified by DPPH (0.88 ± 0.29 mg/mL), ABTS (0.54 ± 0.1 mg/mL) and FRAP assay (26.46 ± 1.65 mg AA/g extract). Ethyl acetate extract have the highest α-amylase (0.09 ± 0.11 mg/mL) and α-glucosidase (0.088 ± 0.13 mg/mL) enzyme inhibition potential. For all the detected molecules, docking and molecular dynamics studies were performed. To the best of our knowledge, this is the first report that discusses LC-ESI-QTOF-MS/MS and GC/QTOF-MS-based metabolites profiling of P. thyrsiformis that can help to treat various diseases.
琴叶珊瑚（P. thyrsiformis）是一种非传统的食用植物，被用作蔬菜和传统药物来治疗各种疾病。这种非传统的食用植物广泛分布于印度、云南-中国省份和越南。尽管有这种潜在的说法，但植物的植物化学研究仍然不完整。在本研究中，使用 LC-ESI-QTOF-MS/MS 和 GC/QTOF-MS 对琴叶珊瑚叶提取物的化学成分进行了分析，并评估了其抗氧化和酶抑制潜力。对不同溶剂提取物的植物代谢产物的初步检查表明，氯仿提取物具有不同的代谢产物，包括酚类、萜类、糖苷和生物碱。使用 LC-ESI-QTOF-MS/MS 和 GC/QTOF-MS 分别鉴定了 113 和 138 种代谢物，包括初级和次级代谢物。氯仿提取物中的总酚含量最高（60.59±1.25 mg GAE/g）。通过 DPPH（0.88±0.29 mg/mL）、ABTS（0.54±0.1 mg/mL）和 FRAP 测定法，发现氯仿提取物具有最高的抗氧化活性（26.46±1.65 mg AA/g 提取物）。乙酸乙酯提取物具有最高的α-淀粉酶（0.09±0.11 mg/mL）和α-葡萄糖苷酶（0.088±0.13 mg/mL）抑制潜力。对所有检测到的分子进行了对接和分子动力学研究。据我们所知，这是首次报道基于 LC-ESI-QTOF-MS/MS 和 GC/QTOF-MS 的琴叶珊瑚代谢产物分析，有助于治疗各种疾病。
