School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Linyi 276006, China.
Biomed Res Int. 2022 Sep 20;2022:1809879. doi: 10.1155/2022/1809879. eCollection 2022.
Jerusalem artichoke ( L.) polysaccharide (JAP) is a chain polysaccharide composed of D-fructose connected by (1-2) glycosidic bonds, which is a kind of inulin. This study evaluated the anti-respiratory syncytial virus (RSV) activity of JAP in and in . To investigate its antiviral activity, an MTT assay, q-PCR, enzyme-linked immunosorbent assay (ELISA), and lung histological observation were performed. The results showed that JAP showed anti-RSV activity in with a half maximal inhibitory concentration (IC) of approximately 29.15 g/mL. results suggested that JAP could effectively inhibit RSV proliferation in the lungs and improve lung tissue lesions in RSV-infected mice. Additionally, JAP could also reduce the expression of TLR3 and TLR4 in the lungs, increase serum anti-inflammatory factors IL-4 levels, and reduce pro-inflammatory factors TNF- and TNF- levels, which may be related to its anti-RSV activity. This study provides a new approach to anti-RSV therapy and enriches the potential applications of JAP.
菊芋（ L.）多糖（JAP）是由通过（1-2）糖苷键连接的 D-果糖组成的链多糖，属于菊糖的一种。本研究评估了 JAP 在 和 中的抗呼吸道合胞病毒（RSV）活性。为了研究其抗病毒活性，进行了 MTT 测定、q-PCR、酶联免疫吸附测定（ELISA）和肺组织学观察。结果表明，JAP 在 中表现出抗 RSV 活性，半数最大抑制浓度（IC）约为 29.15 g/mL。 结果表明，JAP 可有效抑制 RSV 在肺部的增殖并改善 RSV 感染小鼠的肺组织损伤。此外，JAP 还可以降低肺部 TLR3 和 TLR4 的表达，增加血清抗炎因子 IL-4 水平，并降低促炎因子 TNF-和 TNF-水平，这可能与其抗 RSV 活性有关。本研究为抗 RSV 治疗提供了一种新方法，丰富了 JAP 的潜在应用。
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