Agnihotri Saumya, Dobhal Preeti, Tamta Sushma
Department of Botany, D.S.B. Campus, Kumaun University, Nainital, India.
Nat Prod Res. 2023 Aug-Sep;37(16):2759-2764. doi: 10.1080/14786419.2022.2128344. Epub 2022 Oct 6.
The present study carried out to investigate the bioactive chemical compounds, total polyphenol content and antioxidant potential of different extracts of the leaves collected from the Nainital, Uttarakhand. The GC-MS analysis of leaves extract resulted in the isolation of sixty, twelve, twenty-three and nineteen phytochemical constituents in methanol, ethanol, chloroform and water extracts respectively. The leaves extracts were strongly characterised by Heneicosane, Tetratetracontane, Phytol, Fargesin, (+)- Seasmin and Paulowin. Methanol extract showed maximum DPPH (2,2-Di-phenyl-1-picryl-hydrazyl) Free radical scavenging activity (IC 50 15.63 ± 0.31), Ferric Reducing Antioxidant Activity (88.98 ± 3.34 AAE ± SD) and Metal Chelating Activity (IC 50 9.89 ± 0.83). The results showed that the methanolic extract exhibited the highest phenolic content for total phenol content (98.26 ± 0.8 mg of Gallic acid equivalent/g of dry weight), total flavonoid content (61.50 ± 1.62 mg of Quercetin equivalent/g of dry weight) and total tannin content (79.96 ± 0.81 mg of Tannic acid equivalent/g of dry weight).
本研究旨在调查从北阿坎德邦奈尼塔尔采集的树叶不同提取物的生物活性化合物、总多酚含量和抗氧化潜力。树叶提取物的气相色谱 - 质谱分析分别在甲醇、乙醇、氯仿和水提取物中分离出60种、12种、23种和19种植物化学成分。树叶提取物的特征成分主要有二十一烷、四十四烷、叶绿醇、法尔格辛、（+）-芝麻素和保洛文。甲醇提取物表现出最大的2,2 - 二苯基 - 1 - 苦基肼自由基清除活性（IC50为15.63±0.31）、铁还原抗氧化能力（88.98±3.34 AAE±SD）和金属螯合活性（IC50为9.89±0.83）。结果表明，甲醇提取物在总酚含量（98.26±0.8毫克没食子酸当量/克干重）、总黄酮含量（61.50±1.62毫克槲皮素当量/克干重）和总单宁含量（79.96±0.81毫克单宁酸当量/克干重）方面表现出最高的酚类含量。
