Department of Botany, D.S.B. Campus, Kumaun University, Nainital, India.
Nat Prod Res. 2024 Oct;38(19):3404-3410. doi: 10.1080/14786419.2023.2244130. Epub 2023 Aug 11.
The objective of the article is to investigate the chemical composition of roots methanolic extract and to evaluate the polyphenol content and antioxidant potential. Gas Chromatography-Mass Spectroscopy (GC-MS) and High-Performance Liquid Chromatography - Photo Diode Array (HPLC-PDA) techniques were used to characterize chemical composition of methanolic extract of roots. The GC-MS analysis extract resulted in the isolation of thirty-seven phytochemical constituents in methanol extracts. The root extracts were strongly characterized by 1-Propene, 2-nitro-3-(1-cyclooctenyl), (2E,4E)-N-Isobutyldodeca-2,4-dienamide, (+)- Seasmin and Paulowin. The phenolic composition of the root extract quantified by using HPLC revealed Ascorbic acid as most abundant polyphenol, followed by Gallic acid, Chlorogenic acid, Epigallocatechin gallate, Vanillic acid and p-hydroxybenzoic acid, while Caffeic acid was the recorded as least. In addition, root extract also showed the presence of antioxidant activity (DPPH Free radical scavenging, Metal Chelating and Ferric Reducing Antioxidant Activity) and polyphenol content (Total Phenol, Flavonoid, and Tannin Content).
本文旨在研究根甲醇提取物的化学成分，并评估其多酚含量和抗氧化潜力。采用气相色谱-质谱联用（GC-MS）和高效液相色谱-光电二极管阵列（HPLC-PDA）技术对根甲醇提取物的化学成分进行了表征。GC-MS 分析结果表明，甲醇提取物中分离出 37 种植物化学成分。根提取物的特征成分主要有 2-硝基-3-（1-环辛烯基）-1-丙烯、（2E,4E）-N-异丁基十二-2,4-二烯酰胺、（+）-Seasmin 和 Paulowin。采用 HPLC 定量分析根提取物的酚类成分，结果表明抗坏血酸是最丰富的多酚，其次是没食子酸、绿原酸、表没食子儿茶素没食子酸酯、香草酸和对羟基苯甲酸，而咖啡酸则是含量最少的多酚。此外，根提取物还表现出抗氧化活性（DPPH 自由基清除、金属螯合和铁还原抗氧化活性）和多酚含量（总酚、类黄酮和单宁含量）。
